Mouse anti-Human RICTOR Monoclonal Antibody | anti-RICTOR antibody

Mouse Monoclonal [clone 1F3] (IgG2b,k) to Human RICTOR

Cat. Num. AAA246582

• Gene Names: RICTOR; PIA; AVO3; hAVO3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
• Purity: Protein A Purified
• Synonyms: RICTOR; Monoclonal Antibody; Mouse Monoclonal [clone 1F3] (IgG2b; k) to Human RICTOR; Anti-RICTOR Antibody (clone 1F3) IHC-plus; AVO3 homolog; HAVO3; MAVO3; PIA; KIAA1999; TORC2-specific protein AVO3; AVO3; Pianissimo; Human RICTOR; anti-RICTOR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 1F3
• Specificity: Human RICTOR
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.42 mg/ml (varies by lot)
• Sequence Length: 1708

• Applicable Applications for anti-RICTOR antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), IP, WB (1:500 - 1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot using transfected cell lysates, cell line lysates and the recombinant protein used as the immunogen.
• Target Species: Human
• Immunogen Description: RICTOR (NP_689969, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: RICTOR antibody was raised against rICTOR (NP_689969, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-RICTOR antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Western blot of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody (M01), clone 1F3.)

Western Blot (WB)

(RICTOR monoclonal antibody (M01), clone 1F3 Western blot of RICTOR expression in HeLa NE.)

Immunoprecipitation (IP)

(Immunoprecipitation of RICTOR transfected lysate using anti-RICTOR monoclonal antibody and Protein A Magnetic Bead.)

ELISA (EIA)

(Detection limit for recombinant GST tagged RICTOR is approximately 0.03 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 59891448
• NCBI GeneID: 253260
• NCBI Accession #: NP_689969.2
• NCBI GenBank Nucleotide #: NM_152756.4
• UniProt Accession #: Q6R327; Q68DT5; Q86UB7; Q8N3A0; Q8NCM6; B2RNX0; B7ZMF7
• Molecular Weight: 195,011 Da
• NCBI Official Full Name: rapamycin-insensitive companion of mTOR isoform 1
• NCBI Official Synonym Full Names: RPTOR independent companion of MTOR, complex 2
• NCBI Official Symbol: RICTOR
• NCBI Official Synonym Symbols: PIA; AVO3; hAVO3
• NCBI Protein Information: rapamycin-insensitive companion of mTOR; RICTOR; pianissimo; AVO3 homolog; TORC2-specific protein AVO3
• UniProt Protein Name: Rapamycin-insensitive companion of mTOR
• Protein Family: Rapamycin-insensitive companion
• UniProt Gene Name: RICTOR
• UniProt Synonym Gene Names: hAVO3
• UniProt Entry Name: RICTR_HUMAN

NCBI Description

RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]

Uniprot Description

RICTOR: a regulatory binding partner of the kinase mTOR. Part of a rapamycin-insensitive and raptor-independent pathway that regulates the cytoskeleton. Rictor interacts with Cullin1-Rbx1 to form an E3 ubiquitin ligase complex, and promotes ubiquitination and degradation of SGK1. Shares homology with pianissimo from D. discoidieum, STE20p from S. pombe, and AVO3p from S. cerevisiae.

Protein type: Cell cycle regulation; Ubiquitin conjugating system; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5p13.1

Cellular Component: TORC2 complex; cytosol

Molecular Function: protein binding; ribosome binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; TOR signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; regulation of protein kinase B signaling cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-serine phosphorylation; embryonic development; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; actin cytoskeleton reorganization; innate immune response; positive regulation of endothelial cell proliferation; vascular endothelial growth factor receptor signaling pathway; positive regulation of TOR signaling pathway