Western Blot (WB)
(Western blotAll lanes: Annexin A2 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
Rabbit anti-Human Annexin A2 Polyclonal Antibody | anti-ANXA2 antibody
Rabbit anti-human Annexin A2 polyclonal Antibody
Western Blot (WB)
(Western blotAll lanes: Annexin A2 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
Western Blot (WB)
(Western blotAll lanes: Annexin A2 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,PC3 whole cell lysate,U251 whole cell lysate,Mouse lung tissue,Mouse liver tissue,Mouse kidney tissueAll lanes: ANXA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,PC3 whole cell lysate,U251 whole cell lysate,Mouse lung tissue,Mouse liver tissue,Mouse kidney tissueAll lanes: ANXA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung tissue using MBS7000212 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung tissue using MBS7000212 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta using MBS7000212 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human placenta using MBS7000212 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7000212 at dilution of 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using MBS7000212 at dilution of 1:100)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells using MBS7000212 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells using MBS7000212 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunoprecipitation (IP)
(Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7000212 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7000212 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of MBS7000212 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: MBS7000212 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. This gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ANXA2: a calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. Heterotetramer containing 2 light chains of S100A10 2 heavy chains of ANXA2. May cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.
Protein type: Calcium-binding; Lipid-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 15q22.2
Cellular Component: basement membrane; basolateral plasma membrane; cell cortex; cell surface; cytosol; early endosome; endosome; extracellular matrix; extracellular space; extrinsic to plasma membrane; late endosome membrane; lipid particle; lipid raft; lysosomal membrane; melanosome; membrane; midbody; nucleus; perinuclear region of cytoplasm; plasma membrane; ruffle; sarcolemma; vesicle
Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; cytoskeletal protein binding; phosphatidylinositol-4,5-bisphosphate binding; phospholipase A2 inhibitor activity; protease binding; protein binding; Rab GTPase binding; receptor activator activity
Biological Process: angiogenesis; body fluid secretion; collagen fibril organization; fibrinolysis; lipid raft formation; membrane budding; muscle contraction; negative regulation of catalytic activity; negative regulation of low-density lipoprotein receptor catabolic process; negative regulation of receptor internalization; positive regulation of binding; positive regulation of fibroblast proliferation; positive regulation of protein amino acid phosphorylation; positive regulation of vesicle fusion; protein heterotetramerization
Research Articles on ANXA2
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Product Notes
The ANXA2 anxa2 (Catalog #AAA7000212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Annexin A2 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the ANXA2 anxa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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