APOL1 recombinant protein

APOL1 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121573

• Purity: >80% as determined by SDS-PAGE
• Synonyms: APOL1; APOL1 Protein; Human; Recombinant (His Tag); Human APOL1/apolipoprotein L1 Protein (His Tag); APO-L Protein; APOL Protein; APOL-I Protein; FSGS4 Protein; apolipoprotein L1; APOL1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 300mM NaCl, 10%Glycerol, 0.5mM TCEP, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu398

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human APOL1 (Q2KHQ6)(Met1-Leu398) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for APOL1 recombinant protein

Background: APOL1, also known as apolipoprotein L1, is a minor apoprotein component of HDL (High-density lipoprotein) or 'good cholesterol' which is synthesized in the liver and also in many other tissues, including pancreas, kidney, and brain. APOL1 belongs to the apolipoprotein L family. It may play a role in lipid exchange and transport throughout the body. It may also participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

References

Genovese G, et al. (2010) Association of Trypanolytic ApoL1 Variants with Kidney Disease in African-Americans. Science. 329 (5993): 841-5.Tzur S, et al. (2010) Missense mutations in the APOL1 gene are highly associated with end stage kidney disease risk previously attributed to the MYH9 gene. Human Genetics 128 (3): 345-50.Hu CA, et al. (2012) Human apolipoprotein L1 (ApoL1) in cancer and chronic kidney disease. FEBS Lett. 586 (7): 947-55.Papeta N, et al. (2011) APOL1 variants increase risk for FSGS and HIVAN but not IgA nephropathy. J Am Soc Nephrol. 22 (11): 1991-6.Fine DM, et al. (2012) APOL1 risk variants predict histopathology and progression to ESRD in HIV-related kidney disease. J Am Soc Nephrol. 23 (2): 343-50.

NCBI and Uniprot Product Information

• UniProt Accession #: Q2KHQ6
• Molecular Weight: The secreted recombinant human APOL1 consists of 381 amino acids and predicts a molecular mass of 42.5kDa. The apparent molecular mass of the protein is approximately 44kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Family: Apolipoprotein