Apolipoprotein A-I/APOA1 Recombinant Protein | APOA1 recombinant protein

Apolipoprotein A-I/APOA1 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123029

• Gene Names: Apoa1; Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• Purity: >94% as determined by SDS-PAGE
• Synonyms: Apolipoprotein A-I/APOA1; Apolipoprotein A-I/APOA1 Protein; Mouse; Recombinant (His Tag); Mouse ApoA1 Protein (His Tag); 1-Sep Protein; 2-Sep Protein; 42248 Protein; 42249 Protein; Alp-1 Protein; apo-AI Protein; Apoa-1 Protein; apoA-I Protein; Brp-14 Protein; Ltw-1 Protein; Lvtw-1 Protein; apolipoprotein A-I; APOA1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >94% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln264

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Trp 19
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse ApoA1 (Q00623)(Met1-Gln264) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for APOA1 recombinant protein

Background: Apolipoprotein A1 (APOA1) is a member of the apolipoprotein family whose members are proteins bind with lipids and form lipoproteins to translate these oil-soluble lipids such as fat and cholesterol through lymphatic and circulatory system. APOA1 is the main component of high density lipoprotein (HDL) in plasma and is involved in the esterification of cholesterol as a cofactor of lecithin-cholesterol acyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters, and thus play a major role in cholesterol efflux from peripheral cells. As a major component of the HDL complex, APOA1 helps to clear cholesterol from arteries. APOA1 is also characterized as a prostacyclin stabilizing factor, and thus may have an anticlotting effect. Defects in encoding gene may result in HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Men carrying a mutation may develop premature coronary artery disease.

References

Toptas B, et al. (2011) Comparison of lipid profiles with APOA1 MspI polymorphism in obese children with hyperlipidemia. In Vivo. 25(3): 425-30.Haase CL, et al. (2011) Mutation in APOA1 predicts increased risk of ischaemic heart disease and total mortality without low HDL cholesterol levels. J Intern Med. 270(2): 136-46.Wu Z, et al. (2011) The low resolution structure of ApoA1 in spherical high density lipoprotein revealed small angle neutron scattering. J Biol Chem. 286(14): 12495-508.

NCBI and Uniprot Product Information

• NCBI GI #: 160333304
• NCBI GeneID: 11806
• NCBI Accession #: NP_033822.2
• NCBI GenBank Nucleotide #: NM_009692.4
• UniProt Accession #: Q00623; O08855; O09042; Q8BPD5
• Molecular Weight: 30,616 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: Apoa1
• NCBI Official Synonym Symbols: Sep2; Alp-1; Ltw-1; Sep-1; Sep-2; Apoa-1; Brp-14; Lvtw-1; apo-AI; apoA-I
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: Apoa1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_MOUSE

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Cell development/differentiation; Endoplasmic reticulum; Lipid-binding; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Vesicle

Cellular Component: cell surface; chylomicron; cytoplasmic vesicle; endocytic vesicle; extracellular region; extracellular space; nucleus

Molecular Function: apolipoprotein A-I receptor binding; apolipoprotein receptor binding; beta-amyloid binding; chemorepellent activity; cholesterol binding; cholesterol transporter activity; enzyme binding; high-density lipoprotein binding; identical protein binding; lipase inhibitor activity; lipid binding; lipid transporter activity; phosphatidylcholine binding; phospholipid binding; phospholipid transporter activity; protein binding

Biological Process: adrenal gland development; axon regeneration; blood vessel endothelial cell migration; cholesterol biosynthetic process; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; endothelial cell proliferation; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; integrin-mediated signaling pathway; lipoprotein biosynthetic process; negative chemotaxis; negative regulation of cytokine secretion during immune response; negative regulation of heterotypic cell-cell adhesion; negative regulation of hydrolase activity; negative regulation of inflammatory response; negative regulation of interleukin-1 beta secretion; negative regulation of lipase activity; organ regeneration; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phosphatidylcholine metabolic process; phospholipid efflux; phospholipid homeostasis; phospholipid metabolic process; phospholipid transport; positive regulation of fatty acid biosynthetic process; positive regulation of hydrolase activity; positive regulation of lipoprotein lipase activity; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; positive regulation of transferase activity; protein amino acid oxidation; protein localization; protein stabilization; regulation of Cdc42 protein signal transduction; regulation of cholesterol absorption; regulation of protein amino acid phosphorylation; reverse cholesterol transport; sequestering of lipid; triacylglycerol catabolic process; vitamin transport