Dog apolipoprotein A-I ELISA Kit | APOA1 elisa kit

Dog Apolipoprotein A-I, APOA1 ELISA Kit

Cat. Num. AAA943865

• Reactivity: Dog
• Synonyms: apolipoprotein A-I; Dog Apolipoprotein A-I; APOA1 ELISA Kit; Dog Apolipoprotein A-I (APOA1) ELISA kit; MGC117399; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348; APOA1 elisa kit

• Reactivity: Dog
• Specificity: This assay has high sensitivity and excellent specificity for detection of dog APOA1. No significant cross-reactivity or interference between dog APOA1 and analogues was observed.
• Sequence Length: 266

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Competitive
• Detection Range: 28.12 ng/ml -1800 ng/ml.
• Sensitivity: The minimum detectable dose of dog APOA1 is typically less than 7 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA1 elisa kit

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with APOA1. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for APOA1. The competitive inhibition reaction is launched between with pre-coated APOA1 and APOA1 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of APOA1 in the samples. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 3915607
• UniProt Accession #: P02648
• Molecular Weight: 30,196 Da
• NCBI Official Full Name: Apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_CANFA

Uniprot Description

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.