Mouse Apelin ELISA Kit | Apln elisa kit

Mouse Apelin ELISA Kit

Cat. Num. AAA2887050

• Gene Names: Apln; Apel; 6030430G11Rik
• Reactivity: Mouse
• Synonyms: Apelin; Mouse Apelin ELISA Kit; APJ endogenous ligand; Apln; Apel; Apln elisa kit

• Reactivity: Mouse
• Sequence Length: 77

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Apln elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7304895
• NCBI GeneID: 30878
• NCBI Accession #: NP_038940.1
• NCBI GenBank Nucleotide #: NM_013912.3
• UniProt Accession #: Q9R0R4
• Molecular Weight: 8,658 Da
• NCBI Official Full Name: apelin
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: Apln
• NCBI Official Synonym Symbols: Apel; 6030430G11Rik
• NCBI Protein Information: apelin
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: Apln
• UniProt Synonym Gene Names: Apel
• UniProt Entry Name: APEL_MOUSE

Uniprot Description

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; perinuclear region of cytoplasm

Molecular Function: apelin receptor binding; G-protein-coupled receptor binding; hormone activity; receptor binding

Biological Process: drinking behavior; feeding behavior; G-protein coupled receptor protein signaling pathway; negative regulation of blood pressure; negative regulation of vasoconstriction; positive regulation of adrenocorticotropic hormone secretion; positive regulation of cell proliferation; positive regulation of corticotropin-releasing hormone secretion; positive regulation of heat generation; positive regulation of phosphorylation; positive regulation of vasodilation; regulation of body fluid levels; regulation of respiratory gaseous exchange; regulation of the force of heart contraction; signal transduction