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Apoptotic protease-activating factor 1 (APAF1) Recombinant Protein | APAF1 recombinant protein
Recombinant Human Apoptotic protease-activating factor 1 (APAF1) , partial
Gene Names
APAF1; CED4; APAF-1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Apoptotic protease-activating factor 1 (APAF1); Recombinant Human Apoptotic protease-activating factor 1 (APAF1); partial; APAF1 recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
104-415. Partial
Sequence
SGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDIL
Species
Human
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
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Related Product Information for APAF1 recombinant protein
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37.4 kDa
NCBI Official Full Name
apoptotic protease-activating factor 1 isoform b
NCBI Official Synonym Full Names
apoptotic peptidase activating factor 1
NCBI Official Symbol
APAF1
NCBI Official Synonym Symbols
CED4; APAF-1
NCBI Protein Information
apoptotic protease-activating factor 1
UniProt Protein Name
Apoptotic protease-activating factor 1
Protein Family
UniProt Gene Name
APAF1
UniProt Synonym Gene Names
KIAA0413; APAF-1
NCBI Description
This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Research Articles on APAF1
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Product Notes
The APAF1 apaf1 (Catalog #AAA1232957) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 104-415. Partial. The amino acid sequence is listed below: SGITSYVRTV LCEGGVPQRP VVFVTRKKLV NAIQQKLSKL KGEPGWVTIH GMAGCGKSVL AAEAVRDHSL LEGCFPGGVH WVSVGKQDKS GLLMKLQNLC TRLDQDESFS QRLPLNIEEA KDRLRILMLR KHPRSLLILD DVWDSWVLKA FDSQCQILLT TRDKSVTDSV MGPKYVVPVE SSLGKEKGLE ILSLFVNMKK ADLPEQAHSI IKECKGSPLV VSLIGALLRD FPNRWEYYLK QLQNKQFKRI RKSSSYDYEA LDEAMSISVE MLREDIKDYY TDLSILQKDV KVPTKVLCIL WDMETEEVED IL . It is sometimes possible for the material contained within the vial of "Apoptotic protease-activating factor 1 (APAF1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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