Application Data
Human IL-4 ELISA Kit | IL4 elisa kit
Human IL-4 Quick ELISA Kit
Gene Names
IL4; BSF1; IL-4; BCGF1; BSF-1; BCGF-1
Reactivity
Human
Synonyms
IL-4; N/A; Human IL-4 Quick ELISA Kit; IL-4|B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra; IL4 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human IL4
Assay Type
Colorimetric sandwich
Samples
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6pg/ml-1,000pg/ml
Sensitivity
<1.5pg/ml
Capture/Detection Antibodies
Capture antibody- monoclonal antibody from mouse|Detection antibody- polyclonal antibody from goat
Sample Data
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000
O.D.|
0.040|0.148|0.238|0.416|0.755|1.314|2.094|2.570
O.D.|
0.040|0.148|0.238|0.416|0.755|1.314|2.094|2.570
Intra-Assay Precision
Sample|1|2|3|
n|16|16|16|
Mean(pg/ml)|42|224|543|
Standard deviation|2.26|12.09|25.52|
CV(%)|5.4%|5.4%|4.7%|
n|16|16|16|
Mean(pg/ml)|42|224|543|
Standard deviation|2.26|12.09|25.52|
CV(%)|5.4%|5.4%|4.7%|
Inter-Assay Precision
Sample|1|2|3|
n|24|24|24
Mean(pg/ml)|33|199|537
Standard deviation|2.14|10.94|29.53
CV(%)|6.5%|6.6%|5.5%
n|24|24|24
Mean(pg/ml)|33|199|537
Standard deviation|2.14|10.94|29.53
CV(%)|6.5%|6.6%|5.5%
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 42|35|40|33|37|1.59|4.3%
Sample 2: 224|195|213|207|209|8.77|4.2%
Sample 3: 543|445|515|487|497|27.33|5.5%
*number of samples for each test n=16.
Lots: Lot 1 (pg/ml)|Lot 2 (pg/ml)|Lot 3 (pg/ml)|Lot 4 (pg/ml)|Mean (pg/ml)|Standard Deviation|CV (%)
Sample 1: 42|35|40|33|37|1.59|4.3%
Sample 2: 224|195|213|207|209|8.77|4.2%
Sample 3: 543|445|515|487|497|27.33|5.5%
*number of samples for each test n=16.
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Immunogen
Expression system for standard: E Coli; Immunogen sequence: H25-S153
Anticoagulant
heparin or EDTA
Benefits
The Quick ELISA kits, assay takes less than 1.5 hours. Detect Human IL-4 with <1. 5pg/ml sensitivity. Format: 96-well plate with removable strips.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
This is a TMB colorimetric sandwich ELISA kit with short assay time and quick experiment set up.
Principle
Human IL4 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human IL4 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for IL4. Te detection antibody is a HRP linked goat polyclonal antibody specific for IL4. The kit contains recombinant Human IL4 with immunogen: Expression system for standard: E Coli; Immunogen sequence: H25-S153. The kit is analytically validated with ready to use reagents.
To measure Human IL4, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human IL4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL4 in the sample.
To measure Human IL4, add standards and samples to the wells, then add HRP linked detection antibody. Wash the wells with PBS or TBS buffer, and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Human IL4 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human IL4 in the sample.
Assay Procedure Step 1
1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml
TMB Incubation Time
15
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice.)
Application Data
Application Data
Related Product Information for IL4 elisa kit
Interleukin-4(IL-4), also knowns as a B-cell stimulatory factor1(BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells. The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
Product Categories/Family for IL4 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,492 Da
NCBI Official Full Name
interleukin-4 isoform 1
NCBI Official Synonym Full Names
interleukin 4
NCBI Official Symbol
IL4
NCBI Official Synonym Symbols
BSF1; IL-4; BCGF1; BSF-1; BCGF-1
NCBI Protein Information
interleukin-4; binetrakin; pitrakinra; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1
UniProt Protein Name
Interleukin-4
UniProt Gene Name
IL4
UniProt Synonym Gene Names
IL-4; BSF-1
UniProt Entry Name
IL4_HUMAN
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Product Notes
The Human IL4 il4 (Catalog #AAA125853) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA125853 ELISA Kit recognizes Human IL4. It is sometimes possible for the material contained within the vial of "IL-4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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