Annexin A1 (ANXA1) Recombinant Protein | ANXA1 recombinant protein

Recombinant Annexin A1 (ANXA1)

Cat. Num. AAA2011769

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Annexin A1 (ANXA1); Recombinant Annexin A1 (ANXA1); ANXA1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-KGADVNV FTTILTTRSY LHLRRVFQKY SKYSQHDMNK VLDLELKGDI EKCLTAIVQC ATCKPAYFAE KLYQAMKGAG TRHKALIRIM VSRSEVDMND IKAFYQKKYG VSLCQAILDE TKGDYEKILV A
• Sequence Length: 346

• Applicable Applications for ANXA1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Oryctolagus cuniculus (Rabbit)
• Expression System: Prokaryotic expression
• Residues: Lys214~Ala341 (Accession # P51662) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 283837861
• NCBI GeneID: 100328962
• NCBI Accession #: NP_001164623.1
• NCBI GenBank Nucleotide #: NM_001171152.1
• UniProt Accession #: P51662; Q9TQY6
• Molecular Weight: 20.3kDa
• NCBI Official Full Name: annexin A1
• NCBI Official Symbol: ANXA1
• NCBI Protein Information: annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: ANXA1
• UniProt Synonym Gene Names: ANX1
• UniProt Entry Name: ANXA1_RABIT

Uniprot Description

Function: Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. This protein regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity.

Subunit structure: Homodimer in placenta (20%); linked by transglutamylation. Interacts with DYSF

By similarity.

Subcellular location: Nucleus. Cytoplasm. Cell projection › cilium. Basolateral cell membrane. Note: Found in the cilium, nucleus and basolateral cell membrane of ciliated cells in the tracheal endothelium. Found in the cytoplasm of type II pneumocytes and alveolar macrophages. Ref.2

Tissue specificity: In the lung, expressed in the ciliated cells of the tracheal endothelium, but not in the goblet cells. Expressed in type II pneumocytes and alveolar macrophages. Ref.2

Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.

Post-translational modification: Phosphorylated by protein kinase C, epidermal growth factor receptor/kinase and TRPM7. Phosphorylation results in loss of the inhibitory activity

By similarity.

Sequence similarities: Belongs to the annexin family.Contains 4 annexin repeats.