Mitochondrial Antigen
Mitochondrial Antigen
Applications
ELISA
Purity
Affinity PurifiedPurified from bovine spleen and/or thymus through the use of immobilized human anti-Mitochondrial immunoglobulins. Other antigens (Sm, RNP, Scl-70, SS-A(Ro), SS-B(La), Jo-1) are not detected with ELISA assays.
Synonyms
Mitochondrial Antigen; Mitochondrial Antigen antigen
Purity/Purification
Affinity Purified
Purified from bovine spleen and/or thymus through the use of immobilized human anti-Mitochondrial immunoglobulins. Other antigens (Sm, RNP, Scl-70, SS-A(Ro), SS-B(La), Jo-1) are not detected with ELISA assays.
Purified from bovine spleen and/or thymus through the use of immobilized human anti-Mitochondrial immunoglobulins. Other antigens (Sm, RNP, Scl-70, SS-A(Ro), SS-B(La), Jo-1) are not detected with ELISA assays.
Form/Format
Supplied as a frozen liquid in 0.01M Tris, 0.5M sodium chloride, 0.02% sodium azide.
Applicable Applications for Mitochondrial Antigen antigen
ELISA (EL/EIA)
Application Notes
Recommended for use in standard solid phase immunoassays.
Unit Definition
One unit of activity is defined as the amount of antigen that will generate an absorbance of 1.2 when assayed using standard procedure for enzyme immunoassay. Activity will vary with assay conditions.
Preparation and Storage
Product unstable above 0 degree C, aliquot and store frozen at -70 degree C. Avoid repeated freeze-thaw.
Related Product Information for Mitochondrial Antigen antigen
The mitochondrial antigen M2 is a cluster of four major mitochondrial innermembrane proteins of approximately 74, 52, 45, and 39kD, which are found widely in nature. (2) These antigens have been identified as the E2 subunit of branched chain 2-oxo acid dehydrogenase, the E2 subunit of the 2-oxo dehydrogenase complex, protein X, and the E1a and E1b subunits of the pyruvate dehydrogenase complex (3). Autoantibodies to mitochondrial antigens were originally described in patients with Primary Biliary Cirrhosis. It was later realized that Primary Biliary Cirrhosis is an autoimmune liver disease characterized by autoantibodies to the mitochondrial antigen M2 in serum (1).
The presence of autoantibodies against the mitochondrial antigens has been reported to have prognostic value in the identification Primary Biliary Cirrhosis, as the disease rarely occurs without anti-M2. (2) Autoantibodies to M2 in Primary Biliary Cirrhosis usually occur in a very high titer and have an IgG3 subclass restriction (2).
Recent developments in ELISA methology have increased the sensitivity and the capacity of to quantitate patient antibody titers.
The presence of autoantibodies against the mitochondrial antigens has been reported to have prognostic value in the identification Primary Biliary Cirrhosis, as the disease rarely occurs without anti-M2. (2) Autoantibodies to M2 in Primary Biliary Cirrhosis usually occur in a very high titer and have an IgG3 subclass restriction (2).
Recent developments in ELISA methology have increased the sensitivity and the capacity of to quantitate patient antibody titers.
Product Categories/Family for Mitochondrial Antigen antigen
Similar Products
Product Notes
The Mitochondrial Antigen (Catalog #AAA651595) is an Antigen and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Mitochondrial Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Recommended for use in standard solid phase immunoassays. Researchers should empirically determine the suitability of the Mitochondrial Antigen for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondrial Antigen, Antigen" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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