Midkine (NEGF2) Active Protein | NEGF2 active protein

Recombinant Human Midkine (NEGF2)

Cat. Num. AAA4159828

• Gene Names: MDK; MK; ARAP; NEGF2
• Purity: >95% as determined by SDS-PAGE
• Synonyms: Midkine (NEGF2); Recombinant Human Midkine (NEGF2); MK; NEGF-2; NEGF2 active protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution in PBS
• Sequence Length: 143

• Bioactivity: The ED(50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.
• Endotoxin Level: <1.0 EU/ug of recombinant protein as determined by the LAL method.
• Reconstitution: Reconstitute at 0.1 mg/ml in sterile PBS
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at-70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2-8 degree C for one month, or at-20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Testing Data

Testing Data

Related Product Information for NEGF2 active protein

Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.

NCBI and Uniprot Product Information

• NCBI GI #: 396080281
• NCBI GeneID: 4192
• NCBI Accession #: NP_001257480.1
• NCBI GenBank Nucleotide #: NP_001257480.1
• UniProt Accession #: P21741
• Molecular Weight: 14.0 kDa
• NCBI Official Full Name: midkine isoform a
• NCBI Official Synonym Full Names: midkine
• NCBI Official Symbol: MDK
• NCBI Official Synonym Symbols: MK; ARAP; NEGF2
• NCBI Protein Information: midkine
• UniProt Protein Name: Midkine
• UniProt Gene Name: MDK
• UniProt Synonym Gene Names: MK1; NEGF2; MK; ARAP
• UniProt Entry Name: MK_HUMAN

NCBI Description

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

Uniprot Description

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: cell projection; cytoplasm; extracellular region

Molecular Function: heparin binding; growth factor activity

Biological Process: response to drug; nervous system development; cell migration; Notch signaling pathway; dentate gyrus development; behavioral fear response; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; cerebral cortex development; negative regulation of neuron apoptosis; cell differentiation; defecation; regulation of behavior