Testing Data
(Image: Hek293 cell culture transfected with a construct including the C terminal 814-1372 amino acids of Staphylococcus pyogenes Cas9. The cells were stained in red and also with a chicken antibody to S. pyogenes Cas9 in green. A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain)
Cas9 Antibody | anti-Cas9 antibody
Cas9 from S. pyogenes, affinity purified antibody, rabbit
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
Cas9; Cas9 from S. pyogenes; affinity purified antibody; rabbit; anti-Cas9 antibody
Purity/Purification
Affinity Purified
Form/Format
Liquid
Sequence Length
1379
Applicable Applications for anti-Cas9 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Testing Data
(Image: Hek293 cell culture transfected with a construct including the C terminal 814-1372 amino acids of Staphylococcus pyogenes Cas9. The cells were stained in red and also with a chicken antibody to S. pyogenes Cas9 in green. A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain)
Testing Data
(Image: Hek293 cell culture transfected with a construct including the C terminal 814-1372 amino acids of Staphylococcus pyogenes Cas9. The cells were stained in red and also with a chicken antibody to S. pyogenes Cas9 in green. A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain)
Testing Data
(Image: Western blot analysisBlot of 100 ng (lane1) and 10 ng (lane2) of full length Cas9 protein from Streptococcus pyogenes was probed at 1:2,000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.)
Testing Data
(Image: Western blot analysisBlot of 100 ng (lane1) and 10 ng (lane2) of full length Cas9 protein from Streptococcus pyogenes was probed at 1:2,000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.)
Related Product Information for anti-Cas9 antibody
The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has changed the field of gene editing. These repeated sequences are found in bacterial genomes with short DNA sequences derived from viruses which have infected the bacteria interspaced. These virally derived sequences can make short RNA sequences which can hybridize with specific viral DNA and target a nuclease, such as Cas9, to the viral sequence. So, if the bacteria are infected by this virus again, Cas9 can be directed to cleave the specific viral sequence and so inactivate the virus. By careful design of the RNA sequence the system can be used to specifically cut DNA virtually anywhere, including in living human and other mammalian cells. This allows inexpensive gene editing with unprecedented ease, and much effort is going into refining the Cas9 enzymes and their relatives for use in mammalian systems.
Several varieties of Cas9 have been studied and there appear to be several other related enzymes with similar properties in bacteria. Two Cas9 homologs include Streptococcus pyogense and Staphylococcus aureus. Staphylococcus aureus is significantly smaller and so presents less problems when packaged into vectors. The S. pyogenes protein is rather large at 1,368 amino acids, ~160kDa, so the corresponding DNA is also rather large at about 4.2 kb. Our antibody is an affinity purified polyclonal raised in rabbit against a mixture of two recombinant constructs corresponding to amino acids 1-608 and 814-1372 of Cas9 from the Streptococcus pyogenes and binds both these proteins transfected into cells and on western blots, and also recognizes the full length protein. The homologous region of the S. aureus Cas9 is not closely related in amino acid sequence and, as expected, this antibody does not recognize that protein.
Several varieties of Cas9 have been studied and there appear to be several other related enzymes with similar properties in bacteria. Two Cas9 homologs include Streptococcus pyogense and Staphylococcus aureus. Staphylococcus aureus is significantly smaller and so presents less problems when packaged into vectors. The S. pyogenes protein is rather large at 1,368 amino acids, ~160kDa, so the corresponding DNA is also rather large at about 4.2 kb. Our antibody is an affinity purified polyclonal raised in rabbit against a mixture of two recombinant constructs corresponding to amino acids 1-608 and 814-1372 of Cas9 from the Streptococcus pyogenes and binds both these proteins transfected into cells and on western blots, and also recognizes the full length protein. The homologous region of the S. aureus Cas9 is not closely related in amino acid sequence and, as expected, this antibody does not recognize that protein.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Cas9 (Catalog #AAA555742) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cas9 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Researchers should empirically determine the suitability of the Cas9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cas9, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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