Testing Data
(Image: Transfected HEK293 cells overexpressing a fusion protein were stained. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining is shown in red in middle panel. In merged image (right panel), most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.)
Cas9 Antibody | anti-Cas9 antibody
Cas9 from Staphylococcus aureus, antiserum, rabbit
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
Cas9; Cas9 from Staphylococcus aureus; antiserum; rabbit; anti-Cas9 antibody
Form/Format
Whole serum
Sequence Length
1379
Applicable Applications for anti-Cas9 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Testing Data
(Image: Transfected HEK293 cells overexpressing a fusion protein were stained. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining is shown in red in middle panel. In merged image (right panel), most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.)
Testing Data
(Image: Transfected HEK293 cells overexpressing a fusion protein were stained. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining is shown in red in middle panel. In merged image (right panel), most HEK293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.)
Related Product Information for anti-Cas9 antibody
The discovery of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has changed the field of gene editing. These repeated sequences are found in bacterial genomes with short DNA sequences derived from viruses which have infected the bacteria interspaced. These virally derived sequences can make short RNA sequences which can hybridize with specific viral DNA and target a nuclease, such as Cas9, to the viral sequence. So, if the bacteria are infected by this virus again, Cas9 can be directed to cleave the specific viral sequence and so inactivate the virus.
Our antibody is a polyclonal raised in rabbit against the C-terminal 251 amino acids of of the Staphylococcus aureus protein and binds this protein transfected into cells on western blots and in immunocytochemistry. The homologous region of the S. pyogenes is not closely related in amino acid sequence and, as expected, this antibody does not recognize that protein.
Our antibody is a polyclonal raised in rabbit against the C-terminal 251 amino acids of of the Staphylococcus aureus protein and binds this protein transfected into cells on western blots and in immunocytochemistry. The homologous region of the S. pyogenes is not closely related in amino acid sequence and, as expected, this antibody does not recognize that protein.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The Cas9 (Catalog #AAA555743) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Cas9 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the Cas9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cas9, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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