Western Blot (WB)
(Western Blot analysis of A431 and Raji cell using CD2AP Polyclonal Antibody at dilution of 1:1000)
Tight Junction Antibody Kit
Tight Junction Antibody Sampler Kit
Applications
Western Blot
Synonyms
Tight Junction; Tight Junction Antibody Sampler Kit; Tight Junction antibody kit
Applicable Applications for Tight Junction antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of A431 and Raji cell using CD2AP Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of A431 and Raji cell using CD2AP Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of Hela cells using Claudin-1 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using Claudin-1 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using ZO-1 Monoclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of HepG2 cells using ZO-1 Monoclonal Antibody at dilution of 1:2000.)
Related Product Information for Tight Junction antibody kit
Background: Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and basolateral cell surfaces. Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton. The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. Zona occludens proteins ZO-1, -2, and -3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton. ZO-1 and ZO-2 are required for tight junction formation and function. In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation. Exogenous expression of the amino terminal portion of ZO-3 exerts a dominant negative effect that interferes with assembly of tight junctions and adherens junctions. ZO-1 has been shown to interact with afadin prior to the formation of tight junctions. Recent work has also shown that afadin is involved in controlling the directionality of cell movement when it is localized at the leading edge of moving cells. CD2AP is a scaffolding protein that is thought to link membrane proteins to the cytoskeleton. It plays a role in the formation of tight junctions in specialized cell types such as the slit diaphragm of the kidney glomerulus. CD2AP is also involved in the immunological synapse between CD2-expressing T cells and antigen presenting cells. Research studies have shown that interaction between CD2AP and other cytoskeletal proteins may regulate the endocytosis of EGFR.
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Product Notes
The Tight Junction (Catalog #AAA2549975) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Tight Junction can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Tight Junction for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tight Junction, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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