Rabbit anti-Human ZO1 Antibody | anti-ZO1 antibody

ZO1 Antibody

Cat. Num. AAA8502858

• Gene Names: TJP1; ZO-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: ZO1; ZO1 Antibody; TJP1; DKFZp686M05161; MGC133289; ZO-1; anti-ZO1 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 1748

• Applicable Applications for anti-ZO1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:100
• Immunogen: Fusion protein of human ZO1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using NO1 antibody. Immunohistochemistry of paraffin-embeddedH-kidney using NO1antibody)

Related Product Information for anti-ZO1 antibody

Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces (reviewed in 1). Zona occludens proteins ZO-1,-2, and-3 (also known as TJP1, 2, and 3) are peripheral membrane adaptor proteins that link junctional transmembrane proteins such as occludin and claudin to the actin cytoskeleton (reviewed in 2). ZO-1 and-2 are required for tight junction formation and function (3, 4). In subconfluent proliferating cells, ZO-1 and ZO-2 have been shown to colocalize to the nucleus and play a role in transcriptional regulation, possibly through facilitating nuclear import/export of transcriptional regulators (5-7). The ZO-2 gene is transcribed from two promoters, generating the ZO-2A and ZO-2C isoforms. ZO-2C lacks a 23 amino acid amino-terminal sequence found in other ZO-2 isoforms. While both isoforms appear to be widely expressed, abnormal regulation of the ZO-2 gene may be correlated with development of ductal cancer (8).

Product Categories/Family for anti-ZO1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. 2. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11. 3. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47. 4. Umeda, K. et al. (2006) Cell 126, 741-54. 5. Betanzos, A. et al. (2004) Exp Cell Res 292, 51-66. 6. Traweger, A. et al. (2003) J Biol Chem 278, 2692-700. 7. Huerta, M. et al. (2007) Mol Biol Cell 18, 4826-36. 8. Chlenski, A. et al. (2000) Biochim Biophys Acta 1493, 319-24.

NCBI and Uniprot Product Information

• NCBI GI #: 116875767
• NCBI GeneID: 7082
• NCBI Accession #: NP_003248.3
• NCBI GenBank Nucleotide #: NM_003257.4
• UniProt Accession #: Q07157; Q2NKP3; Q4ZGJ6; B4E3K1
• Molecular Weight: 195kDa
• NCBI Official Full Name: tight junction protein ZO-1 isoform a
• NCBI Official Synonym Full Names: tight junction protein 1
• NCBI Official Symbol: TJP1
• NCBI Official Synonym Symbols: ZO-1
• NCBI Protein Information: tight junction protein ZO-1
• UniProt Protein Name: Tight junction protein ZO-1
• UniProt Gene Name: TJP1
• UniProt Synonym Gene Names: ZO1

NCBI Description

This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Uniprot Description

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus ().