SAPK/JNK antibody kit

Phospho-SAPK/JNK Pathway Antibody Sampler Kit

Cat. Num. AAA2549991

• Applications: Western Blot
• Synonyms: SAPK/JNK; Phospho-SAPK/JNK Pathway Antibody Sampler Kit; SAPK/JNK antibody kit

• Applicable Applications for SAPK/JNK antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of KB, MCF7 cells with Phospho-JNK1/2/3 (Thr183/Y185) Polyclonal Antibody at dilution of 1:2000)

Western Blot (WB)

(Western Blot analysis of 293 cells with Phospho-MEK-4 (Thr261) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of HuvEc cells with Phospho-ATF-2 (Thr71) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB)

(Western Blot analysis of Hela cells with Phospho-AP-1 (Ser63) Polyclonal Antibody at dilution of 1:1000)

Related Product Information for SAPK/JNK antibody kit

Background: The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase. Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member. There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.