Rabbit MCL1 Antibody | anti-MCL1 antibody

MCL1 Antibody

Cat. Num. AAA8502146

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purification
• Synonyms: MCL1; MCL1 Antibody; BCL2L3; EAT; MCL1L; MCL1S; MGC104264; MGC1839; Mcl-1; TM; anti-MCL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 197

• Applicable Applications for anti-MCL1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: C term-peptide of human MCL1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MCL1 antibody.)

Related Product Information for anti-MCL1 antibody

Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway (1). Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria (2), interacts with and antagonizes pro-apoptotic Bcl-2 family members (3), and inhibits apoptosis induced by a number of cytotoxic stimuli (4). Mcl-1 differs from its other family members in its regulation at both the transcriptional and post-translational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life (1, 2). Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation (1, 5-7). Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal (8-11). Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover (10) but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization (11). Mcl-1 deficiency in mice results in peri-implantation lethality (12). In addition, conditional disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes (13).

Product Categories/Family for anti-MCL1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Kozopas, K.M. et al. (1993) Proc Natl Acad Sci USA 90, 3516-20. 2. Yang, T. et al. (1995) J Cell Biol 128, 1173-84. 3. Sato, T. et al. (1994) Proc Natl Acad Sci USA 91, 9238-42. 4. Zhou, P. et al. (1997) Blood 89, 630-43. 5. Wang, J.M. et al. (1999) Mol Cell Biol 19, 6195-206. 6. Jourdan, M. et al. (2003) Oncogene 22, 2950-9. 7. Chao, J.R. et al. (1998) Mol Cell Biol 18, 4883-98. 8. Domina, A.M. et al. (2000) J Biol Chem 275, 21688-94. 9. Inoshita, S. et al. (2002) J Biol Chem 277, 43730-4. 10. Domina, A.M. et al. (2004) Oncogene 23, 5301-15. 11. Maurer, U. et al. (2006) Mol Cell 21, 749-60. 12. Rinkenberger, J.L. et al. (2000) Genes Dev 14, 23-7. 13. Opferman, J.T. et al. (2003) Nature 426, 671-6.

NCBI and Uniprot Product Information

• NCBI GI #: 309747067
• NCBI GeneID: 4170
• NCBI Accession #: NP_001184249.1
• NCBI GenBank Nucleotide #: NM_001197320.1
• UniProt Accession #: Q07820; Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
• Molecular Weight: 37kDa
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
• NCBI Official Synonym Full Names: MCL1, BCL2 family apoptosis regulator
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• Protein Family: Minichromosome loss protein
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3

NCBI Description

This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing. [provided by RefSeq, Oct 2010]

Uniprot Description

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.