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Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Chk2 (Thr68) Polyclonal Antibody at dilution of 1:500)
DNA Damage Antibody Kit
DNA Damage Antibody Sampler Kit
Applications
Western Blot
Synonyms
DNA Damage; DNA Damage Antibody Sampler Kit; DNA Damage antibody kit
Applicable Applications for DNA Damage antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Chk2 (Thr68) Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Chk2 (Thr68) Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Histone H2AX (Ser139) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of Hela cells with Phospho-Histone H2AX (Ser139) Polyclonal Antibody at dilution of 1:2000)
Western Blot (WB)
(Western Blot analysis of HT-29 cells with Phospho-p53 (Ser15) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of HT-29 cells with Phospho-p53 (Ser15) Polyclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of K562 cells with Phospho-Atm (Ser1981) Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of K562 cells with Phospho-Atm (Ser1981) Polyclonal Antibody at dilution of 1:500)
Related Product Information for DNA Damage antibody kit
Background: Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 Kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks. p53 is phosphorylated by ATM, ATR and DNA-PK at Ser15. This phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage. Chk1 and Chk2, downstream protein kinases of ATM/ATR, plays an important role in DNA damage checkpoint control, embryonic development and tumor suppression. Chk1 is phosphorylated at Ser280 and Ser296 following DNA damage. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues, including Thr68, each followed by glutamine (SQ or TQ motif). After DNA damage by ionizing radiation (IR), UV irradiation or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The breast cancer susceptibility proteins BRCA1 and BRCA2 are frequently mutated in cases of hereditary breast and ovarian cancers and have roles in multiple processes related to DNA damage, repair, cell cycle progression, transcription, ubiquitination and apoptosis. Numerous DNA-damage induced phosphorylation sites on BRCA1 have been identified, including serine 1524, and kinases activated in a cell cycle-dependent manner, including Aurora A and CDK2, can also phosphorylate BRCA1. IR, DNA and radiometric-induced DNA damage also results in rapid phosphorylation of the histone H2A family member H2A.X at Ser139 by ATM. Within minutes following DNA damage, Ser139-phosphorylated H2A.X localizes to sites of DNA damage at subnuclear foci.
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Product Notes
The DNA Damage (Catalog #AAA2550089) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's DNA Damage can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DNA Damage for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Damage, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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