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Western Blot (WB)
(Western Blot analysis of K562 cells with Phospho-Atm (Ser1981) Polyclonal Antibody at dilution of 1:500)
Double Strand Breaks (DSB) Antibody Kit | DSB antibody kit
Double Strand Breaks (DSB) Repair Antibody Sampler Kit
Applications
Western Blot
Synonyms
Double Strand Breaks (DSB); Double Strand Breaks (DSB) Repair Antibody Sampler Kit; DSB antibody kit
Applicable Applications for DSB antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of K562 cells with Phospho-Atm (Ser1981) Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of K562 cells with Phospho-Atm (Ser1981) Polyclonal Antibody at dilution of 1:500)
Western Blot (WB)
(Western Blot analysis of Mouse brain, K562 using Atm Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of Mouse brain, K562 using Atm Polyclonal Antibody at dilution of 1:500.)
Western Blot (WB)
(Western Blot analysis of Hela cells using DNA-PKCS Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using DNA-PKCS Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of K562, Jurkat cells with Ku-80 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of K562, Jurkat cells with Ku-80 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of K562 cells with MRE11 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of K562 cells with MRE11 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using Rad50 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of COLO205 cells using Rad50 Polyclonal Antibody at dilution of 1:1000.)
Related Product Information for DSB antibody kit
Background: Double strand DNA breaks (DSB) in mammalian cells can be repaired by the related mechanisms of non-homologous end-joining (NHEJ) and homologous recombination (HR). A DNA-dependent protein kinase composed of DNA-binding subunits Ku70 and Ku86 and the DNA-PKcs catalytic subunit mediates NHEJ repair. The Ku heterodimer binds free DNA ends and recruits DNA-PKcs to the break. DNA-PKcs signals areas of DNA damage and recruits additional proteins, such as the Artemis exo- and endonuclease that processes and primes the damaged sequence. Following replacement DNA synthesis, a ligase complex composed of DNA ligase IV and XRCC4 joins the repaired ends. XRCC4-like factor (XLF) is an essential ligase-associated repair factor that promotes gap- filling during NHEJ. Homologous recombination utilizes aligned homologous sequences as a repair template. The MRN complex, composed of Mre11, Rad50, and nibrin (p95/NBS1), plays a critical role in sensing, processing and repairing breaks. MRN interacts with BRCA1 and CtIP to facilitate 5' resection of DSB DNA to generate 3' ssDNA ends necessary for repair. DNA-binding protein Mre11 exhibits exonuclease and endonuclease activity and is largely responsible for ssDNA end processing. Interaction between the MRN complex and ATM kinase promotes association between the kinase and its substrates and likely leads to ATM activation. ATM acts a central controller of the cell cycle checkpoint by phosphorylating multiple targets, including c-Abl, BRCA1 and p95/NSB1. Activated c-Abl phosphorylates Rad52, which promotes Rad51 binding to ssDNA and subsequent annealing of ssDNA.
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Product Notes
The DSB (Catalog #AAA2549997) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Double Strand Breaks (DSB) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the DSB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Double Strand Breaks (DSB), Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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