Typical Testing Data/Standard Curve (for reference only)
Sheep Angiogenin ELISA Kit | ANG elisa kit
Sheep Angiogenin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This ANG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ANG. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ANG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANG antibody and an ANG-HRP conjugate. The assay sample and buffer are incubated together with ANG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANG concentration since ANG from samples and ANG-HRP conjugate compete for the anti-ANG antibody binding site. Since the number of sites is limited, as more sites are occupied by ANG from the sample, fewer sites are left to bind ANG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANG concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.
Protein type: RNA-binding; Motility/polarity/chemotaxis; EC 3.1.27.-; Ribonuclease; Cell cycle regulation; Secreted, signal peptide; Nucleolus; Extracellular matrix; Secreted
Chromosomal Location of Human Ortholog: 14q11.1-q11.2
Cellular Component: angiogenin-PRI complex; extracellular space; growth cone; cell soma; nucleolus; basal lamina; nucleus
Molecular Function: heparin binding; rRNA binding; protein binding; copper ion binding; DNA binding; endonuclease activity; actin binding; peptide binding; receptor binding; ribonuclease activity
Biological Process: cell migration; negative regulation of smooth muscle cell proliferation; diacylglycerol biosynthetic process; activation of protein kinase B; activation of phospholipase A2; response to hormone stimulus; oocyte maturation; rRNA transcription; ovarian follicle development; homeostatic process; positive regulation of protein secretion; actin filament polymerization; phospholipase C activation; negative regulation of translation; response to hypoxia; positive regulation of endothelial cell proliferation; cell communication; angiogenesis; positive regulation of phosphorylation; placenta development
Disease: Amyotrophic Lateral Sclerosis 9
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Product Notes
The Sheep ANG ang (Catalog #AAA741402) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741402 ELISA Kit recognizes Sheep ANG. It is sometimes possible for the material contained within the vial of "Angiogenin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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