Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit | SLPI elisa kit

Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

Cat. Num. AAA2703160

• Gene Names: SLPI; ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
• Reactivity: Human
• Synonyms: Secretory Leukocyte Peptidase Inhibitor (SLPI); Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit; ALK1; ALP; MPI; BLPI; HUSI; HUSI-I; WAP4; WFDC4; Antileukoproteinase; Seminal proteinase inhibitor; Mucus proteinase inhibitor; WAP four-disulfide core domain protein 4; SLPI elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Secretory Leukocyte Peptidase Inhibitor (SLPI).; No significant cross-reactivity or interference between Secretory Leukocyte Peptidase Inhibitor (SLPI) and analogues was observed.
• Sequence Length: 132

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 62.5-4,000pg/mL
• Sensitivity: < 27.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretory Leukocyte Peptidase Inhibitor (SLPI) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLPI elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretory Leukocyte Peptidase Inhibitor (SLPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretory Leukocyte Peptidase Inhibitor (SLPI), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secretory Leukocyte Peptidase Inhibitor (SLPI) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119596275
• NCBI GeneID: 6590
• UniProt Accession #: P03973; P07757; B2R5H8
• Molecular Weight: 14,326 Da
• NCBI Official Full Name: secretory leukocyte peptidase inhibitor
• NCBI Official Synonym Full Names: secretory leukocyte peptidase inhibitor
• NCBI Official Symbol: SLPI
• NCBI Official Synonym Symbols: ALP; MPI; ALK1; BLPI; HUSI; WAP4; WFDC4; HUSI-I
• NCBI Protein Information: antileukoproteinase
• UniProt Protein Name: Antileukoproteinase
• Protein Family: Antileukoproteinase
• UniProt Gene Name: SLPI
• UniProt Synonym Gene Names: WAP4; WFDC4; MPI

NCBI Description

This gene encodes a secreted inhibitor which protects epithelial tissues from serine proteases. It is found in various secretions including seminal plasma, cervical mucus, and bronchial secretions, and has affinity for trypsin, leukocyte elastase, and cathepsin G. Its inhibitory effect contributes to the immune response by protecting epithelial surfaces from attack by endogenous proteolytic enzymes. This antimicrobial protein has antibacterial, antifungal and antiviral activity. [provided by RefSeq, Nov 2014]

Uniprot Description

Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:3533531, PubMed:3462719, PubMed:2039600, PubMed:2110563, PubMed:10702419, PubMed:24121345). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:10702419, PubMed:24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed:24352879).