Rat Angiogenin ELISA Kit | ANG elisa kit

Rat Angiogenin ELISA Kit

Cat. Num. AAA765401

• Gene Names: ANG; ALS9; RAA1; HEL168; RNASE4; RNASE5
• Reactivity: Rat
• Synonyms: Angiogenin; Rat Angiogenin ELISA Kit; ANG (Angiogenin)/ALS9/HEL168/RNASE4/RNASE5; ANG elisa kit

• Reactivity: Rat
• Sequence Length: 147

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 37.5pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ANG elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat ANG antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat ANG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat ANG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat ANG can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 148277046
• NCBI GeneID: 283
• NCBI Accession #: NP_001091046.1
• NCBI GenBank Nucleotide #: NM_001097577.2
• Molecular Weight: 16,550 Da
• NCBI Official Full Name: angiogenin
• NCBI Official Synonym Full Names: angiogenin, ribonuclease, RNase A family, 5
• NCBI Official Symbol: ANG
• NCBI Official Synonym Symbols: ALS9; RAA1; HEL168; RNASE4; RNASE5
• NCBI Protein Information: angiogenin
• UniProt Protein Name: Angiogenin
• Protein Family: Angiogenin
• UniProt Gene Name: ANG
• UniProt Synonym Gene Names: RNASE5; RNase 5
• UniProt Entry Name: ANGI_HUMAN

NCBI Description

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]

Uniprot Description

ANG: May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9). ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. Belongs to the pancreatic ribonuclease family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Extracellular matrix; Ribonuclease; Cell cycle regulation; EC 3.1.27.-; RNA-binding; Nucleolus; Secreted

Chromosomal Location of Human Ortholog: 14q11.1-q11.2

Cellular Component: angiogenin-PRI complex; basal lamina; cell soma; cytoplasmic membrane-bound vesicle; extracellular region; extracellular space; growth cone; nucleolus; nucleus

Molecular Function: actin binding; copper ion binding; DNA binding; endonuclease activity; heparin binding; peptide binding; protein binding; protein homodimerization activity; receptor binding; ribonuclease activity; rRNA binding

Biological Process: actin filament polymerization; activation of phospholipase A2; activation of protein kinase B; angiogenesis; antibacterial humoral response; antifungal humoral response; cell communication; cell migration; defense response to Gram-positive bacterium; diacylglycerol biosynthetic process; homeostatic process; innate immune response; intercellular junction assembly and maintenance; negative regulation of smooth muscle cell proliferation; negative regulation of translation; oocyte maturation; ovarian follicle development; phospholipase C activation; placenta development; positive regulation of endothelial cell proliferation; positive regulation of phosphorylation; positive regulation of protein secretion; response to hormone stimulus; response to hypoxia; response to yeast; rRNA transcription

Disease: Amyotrophic Lateral Sclerosis 9