Human angiogenin, ribonuclease, RNase A family, 5 ELISA Kit | ANG elisa kit

Human Angiogenin, ANG ELISA Kit

Cat. Num. AAA704672

• Gene Names: ANG; ALS9; RAA1; HEL168; RNASE4; RNASE5
• Reactivity: Human
• Synonyms: angiogenin; ribonuclease; RNase A family; 5; Human Angiogenin; ANG ELISA Kit; ALS9; HEL168; MGC22466; MGC71966; RNASE4; RNASE5; OTTHUMP00000164021; epididymis luminal protein 168; ANG; ANG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ANG. No significant cross-reactivity or interference between Human ANG and analogues was observed.
• Sequence Length: 147

• Samples: Serum, plasma, cell culture supernates, urine, tissue homogenates
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: 69.915 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cardiovascular
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANG elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ANG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ANG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ANG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ANG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148277046
• NCBI GeneID: 283
• NCBI Accession #: NP_001091046.1
• NCBI GenBank Nucleotide #: NM_001097577.2
• UniProt Accession #: P03950; Q05CV1; Q53X86; Q6P5T2; Q8WXE7
• Molecular Weight: 16,550 Da
• NCBI Official Full Name: angiogenin
• NCBI Official Synonym Full Names: angiogenin, ribonuclease, RNase A family, 5
• NCBI Official Symbol: ANG
• NCBI Official Synonym Symbols: ALS9; RAA1; HEL168; RNASE4; RNASE5
• NCBI Protein Information: angiogenin; RNase 5; ribonuclease 5; ribonuclease A A1; epididymis luminal protein 168
• UniProt Protein Name: Angiogenin
• Protein Family: Angiogenin
• UniProt Gene Name: ANG
• UniProt Synonym Gene Names: RNASE5; RNase 5
• UniProt Entry Name: ANGI_HUMAN

NCBI Description

The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region. [provided by RefSeq, Aug 2014]

Uniprot Description

Function: Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Stimulates ribosomal RNA synthesis including that containing the initiation site sequences of 45S rRNA. Cleaves tRNA within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs) which inhibit protein synthesis and triggers the assembly of stress granules (SGs). Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo.4 PublicationsManual assertion based on experiment in:Ref.10