Rabbit BLZF1 Polyclonal Antibody | anti-BLZF1 antibody

BLZF1 Polyclonal Antibody

Cat. Num. AAA3007824

• Gene Names: BLZF1; JEM1; JEM-1; JEM-1s; GOLGIN-45
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: BLZF1; Polyclonal Antibody; BLZF1 Polyclonal Antibody; Golgin-45; Basic leucine zipper nuclear factor 1; JEM-1; p45 basic leucine-zipper nuclear factor; JEM1; anti-BLZF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BLZF1 polyclonal antibody detects endogenous levels of BLZF1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 400

• Applicable Applications for anti-BLZF1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human BLZF1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of BLZF1 polyclonal antibody)

Related Product Information for anti-BLZF1 antibody

Golgin 45, also known as JEM-1 or Basic Leucine Zipper Nuclear Factor 1, is a 400 amino acid protein that localizes to the lumen of the golgi apparatus. The primary function of golgin 45 is to maintain normal golgi structure and to facilitate protein transport from the endoplasmic reticulum to the cell surface. The latter process is regulated primarily through the interaction of golgin 45 and GRASP55, which, together, form a Rab 2 effector complex.Excluding formation of a Rab 2 complex, golgin 45 does not interact with any other golgi Rab proteins. Golgin 45 is expressed as two isoforms, one of which localizes to the nucleus and is thought to function as a transcription factor.

NCBI and Uniprot Product Information

• NCBI GI #: 4504805
• NCBI GeneID: 8548
• NCBI Accession #: NP_003657.1
• NCBI GenBank Nucleotide #: NP_003657.1
• UniProt Accession #: Q9H2G9
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: golgin-45 isoform 1
• NCBI Official Synonym Full Names: basic leucine zipper nuclear factor 1
• NCBI Official Symbol: BLZF1
• NCBI Official Synonym Symbols: JEM1; JEM-1; JEM-1s; GOLGIN-45
• NCBI Protein Information: golgin-45
• UniProt Protein Name: Golgin-45
• Protein Family: Golgin
• UniProt Gene Name: BLZF1
• UniProt Synonym Gene Names: JEM1
• UniProt Entry Name: GO45_HUMAN

Uniprot Description

BLZF1: Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1q24

Cellular Component: nucleoplasm; Golgi membrane; Golgi lumen; cytoplasm; nucleus

Molecular Function: enzyme binding; DNA binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; cell proliferation; Golgi to plasma membrane protein transport; mitotic cell cycle; regulation of cell growth; Golgi organization and biogenesis