Porcine Adrenomedullin (ADM) ELISA Kit | ADM elisa kit

Porcine Adrenomedullin (ADM) ELISA Kit

Cat. Num. AAA2019920

• Gene Names: ADM; AM; PAMP
• Reactivity: Porcine
• Synonyms: Adrenomedullin (ADM); Porcine Adrenomedullin (ADM) ELISA Kit; AM; PAMP; Proadrenomedullin N-20 terminal peptide; ProAM N-terminal 20 peptide; ADM elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adrenomedullin (ADM).; No significant cross-reactivity or interference between Adrenomedullin (ADM) and analogues was observed.
• Sequence Length: 185

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 12.35-1000pg/mL
• Sensitivity: < 4.85pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adrenomedullin (ADM) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADM elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adrenomedullin (ADM) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adrenomedullin (ADM) and unlabeled Adrenomedullin (ADM) (Standards or samples) with the pre-coated antibody specific to Adrenomedullin (ADM). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adrenomedullin (ADM) in the sample.

Product Categories/Family for ADM elisa kit

Endocrinology; Hormone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 765330
• NCBI GeneID: 133
• UniProt Accession #: P35318; Q6FGW2; B2R793; D3DQV3
• Molecular Weight: 20,420 Da
• NCBI Official Full Name: adrenomedullin
• NCBI Official Synonym Full Names: adrenomedullin
• NCBI Official Symbol: ADM
• NCBI Official Synonym Symbols: AM; PAMP
• NCBI Protein Information: ADM
• UniProt Protein Name: ADM
• Protein Family: ADM
• UniProt Gene Name: ADM
• UniProt Synonym Gene Names: AM; AM; PAMP; ProAM-N20
• UniProt Entry Name: ADML_HUMAN

NCBI Description

The protein encoded by this gene is a preprohormone which is cleaved to form two biologically active peptides, adrenomedullin and proadrenomedullin N-terminal 20 peptide. Adrenomedullin is a 52 aa peptide with several functions, including vasodilation, regulation of hormone secretion, promotion of angiogenesis, and antimicrobial activity. The antimicrobial activity is antibacterial, as the peptide has been shown to kill E. coli and S. aureus at low concentration. [provided by RefSeq, Aug 2014]

Uniprot Description

ADM: AM and PAMP are potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, am is diuretic and natriuretic, and both am and pamp inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Belongs to the adrenomedullin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: extracellular space; cytoplasm; extracellular region

Molecular Function: protein binding; adrenomedullin receptor binding; hormone activity; receptor binding

Biological Process: developmental growth; blood circulation; positive regulation of apoptosis; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; female pregnancy; positive regulation of vasodilation; signal transduction; response to insulin stimulus; negative regulation of cell proliferation; elevation of cytosolic calcium ion concentration; cAMP-mediated signaling; cell-cell signaling; receptor internalization; positive regulation of cell proliferation; positive regulation of cAMP biosynthetic process; response to wounding; vasculogenesis; regulation of the force of heart contraction; aging; hormone secretion; progesterone biosynthetic process; organ regeneration; androgen metabolic process; neurite regeneration; positive regulation of heart rate; negative regulation of vasoconstriction; odontogenesis of dentine-containing teeth; response to starvation; positive regulation of angiogenesis; G-protein coupled receptor internalization; neural tube closure; response to hypoxia; negative regulation of vascular permeability; cAMP biosynthetic process; response to cold