Amelogenin, X-Linked (AMELX) Recombinant Protein | AMELX recombinant protein

Recombinant Amelogenin, X-Linked (AMELX)

Cat. Num. AAA2010569

• Gene Names: AMELX; AMG; AI1E; AIH1; ALGN; AMGL; AMGX
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Amelogenin; X-Linked (AMELX); Recombinant Amelogenin; AMELX recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- YP SYGYEPMGGW LHHQIIPVLS QQHPPTHTLQ PHHHIPVVPA QQPVIPQQPM MPVPGQHSMT PIQHHQPNLP PPAQQPYQPQ PVQPQPHQPM QPQPPVHPMQ PLPPQPPLPP MFPMQPLPPM LPDLTLEAWP STDKTKR
• Sequence Length: 191

• Applicable Applications for AMELX recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Tyr49~Arg187 (Accession # Q99217) with N-terminal His-Tag
• Subcellular Location: Secreted, extracellular space, extracellular matrix
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.8
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4502071
• NCBI GeneID: 265
• NCBI Accession #: NP_001133.1
• NCBI GenBank Nucleotide #: NM_001142.2
• UniProt Accession #: Q99217; Q96NW6; Q9UCA7
• Molecular Weight: 17.3kDa
• NCBI Official Full Name: amelogenin, X isoform isoform 1
• NCBI Official Synonym Full Names: amelogenin, X-linked
• NCBI Official Symbol: AMELX
• NCBI Official Synonym Symbols: AMG; AI1E; AIH1; ALGN; AMGL; AMGX
• NCBI Protein Information: amelogenin, X isoform; amelogenesis imperfecta 1; amelogenin (amelogenesis imperfecta 1, X-linked); amelogenin (X chromosome, amelogenesis imperfecta 1)
• UniProt Protein Name: Amelogenin, X isoform
• Protein Family: Amelogenin
• UniProt Gene Name: AMELX
• UniProt Synonym Gene Names: AMG; AMGX
• UniProt Entry Name: AMELX_HUMAN

NCBI Description

This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

AMELX: Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel. Defects in AMELX are the cause of amelogenesis imperfecta type 1E (AI1E). A X-linked defect of dental enamel formation. Teeth have only a thin layer of enamel with normal hardness. The thinness of the enamel makes the teeth appear small. Belongs to the amelogenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xp22.31-p22.1

Cellular Component: proteinaceous extracellular matrix; cell surface

Molecular Function: identical protein binding; protein binding; growth factor activity; hydroxyapatite binding; structural constituent of tooth enamel

Biological Process: osteoblast differentiation; cell proliferation; biomineral formation; ion homeostasis; positive regulation of collagen biosynthetic process; chondrocyte differentiation; epithelial to mesenchymal transition; signal transduction; cell adhesion; odontogenesis of dentine-containing teeth

Disease: Amelogenesis Imperfecta, Type Ie