Human Amelogenin, X isoform ELISA Kit | AMELX elisa kit

Human Amelogenin, X isoform ELISA Kit

Cat. Num. AAA763849

• Gene Names: AMELX; AMG; AI1E; AIH1; ALGN; AMGL; AMGX
• Reactivity: Human
• Synonyms: Amelogenin; X isoform; Human Amelogenin; X isoform ELISA Kit; AMELX/Amelogenin; X isoform/AMG/AMGX/AIH1/ALGN/AMGL; AMELX elisa kit

• Reactivity: Human
• Sequence Length: 191

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AMELX elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human AMELXantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human AMELX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human AMELX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman AMELXcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502071
• NCBI GeneID: 265
• NCBI Accession #: NP_001133.1
• NCBI GenBank Nucleotide #: NM_001142.2
• Molecular Weight: 23,111 Da
• NCBI Official Full Name: amelogenin, X isoform isoform 1
• NCBI Official Synonym Full Names: amelogenin, X-linked
• NCBI Official Symbol: AMELX
• NCBI Official Synonym Symbols: AMG; AI1E; AIH1; ALGN; AMGL; AMGX
• NCBI Protein Information: amelogenin, X isoform
• UniProt Protein Name: Amelogenin, X isoform
• Protein Family: Amelogenin
• UniProt Gene Name: AMELX
• UniProt Synonym Gene Names: AMG; AMGX
• UniProt Entry Name: AMELX_HUMAN

NCBI Description

This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

AMELX: Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel. Defects in AMELX are the cause of amelogenesis imperfecta type 1E (AI1E). A X-linked defect of dental enamel formation. Teeth have only a thin layer of enamel with normal hardness. The thinness of the enamel makes the teeth appear small. Belongs to the amelogenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp22.31-p22.1

Cellular Component: cell surface; proteinaceous extracellular matrix

Molecular Function: growth factor activity; hydroxyapatite binding; identical protein binding; protein binding; structural constituent of tooth enamel

Biological Process: biomineral formation; cell adhesion; cell proliferation; chondrocyte differentiation; epithelial to mesenchymal transition; ion homeostasis; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of collagen biosynthetic process; signal transduction

Disease: Amelogenesis Imperfecta, Type Ie