Mouse Ornithine Decarboxylase-1 (ODC-1) Antibody | anti-ODC-1 antibody

Ornithine Decarboxylase-1 (ODC-1)

Cat. Num. AAA4381137

• Gene Names: ODC1; ODC
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: Ornithine Decarboxylase-1 (ODC-1); Ornithine decarboxylase structural 1; RNODC; anti-ODC-1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Isotype: IgG1, kappa
• Clone Number: rODC1/485
• Specificity: Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 461

• Applicable Applications for anti-ODC-1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Human placenta or prostate carcinoma tissue (IHC).
• Immunogen: Recombinant full-length human ODC-1 protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 2p25.1
• Antibody Type: Recombinant Mouse Monoclonal Antibody
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified ODC-1 Recombinant Mouse Monoclonal Antibody (rODC1/485). Confirmation of Purity and Integrity of Antibody.)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human prostate carcinoma stained with ODC-1 Recombinant Mouse Monoclonal Antibody (rODC1/485).)

References

Schipper RG; et al. Journal of Immunological Methods, 1993, 161(2):205-15.

NCBI and Uniprot Product Information

• NCBI GI #: 4505489
• NCBI GeneID: 4953
• NCBI Accession #: NP_002530.1
• NCBI GenBank Nucleotide #: NP_002530.1
• UniProt Accession #: P11926
• Molecular Weight: 53kDa
• NCBI Official Full Name: ornithine decarboxylase isoform 1
• NCBI Official Synonym Full Names: ornithine decarboxylase 1
• NCBI Official Symbol: ODC1
• NCBI Official Synonym Symbols: ODC
• NCBI Protein Information: ornithine decarboxylase
• UniProt Protein Name: Ornithine decarboxylase
• UniProt Gene Name: ODC1
• UniProt Synonym Gene Names: ODC
• UniProt Entry Name: DCOR_HUMAN

NCBI Description

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Dec 2013]

Uniprot Description

ODC: the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter. [provided by RefSeq, Jul 2008]

Protein type: EC 4.1.1.17; Amino Acid Metabolism - arginine and proline; Other Amino Acids Metabolism - glutathione; Cell cycle regulation; Lyase

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: cytoplasm; cytosol

Molecular Function: protein homodimerization activity; ornithine decarboxylase activity

Biological Process: response to virus; positive regulation of cell proliferation; regulation of protein catabolic process; kidney development; regulation of amino acid metabolic process; putrescine biosynthetic process from ornithine; polyamine metabolic process

Disease: Colorectal Cancer