Related Product Information for Amelx elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Amelx antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Amelx antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Amelx amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Amelx can be calculated.
NCBI Description
This gene encodes a member of the amelogenin family of extracellular matrix proteins. Amelogenins are involved in biomineralization during tooth enamel development. Mutations in this gene cause X-linked amelogenesis imperfecta. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
AMELX: Plays a role in biomineralization. Seems to regulate the formation of crystallites during the secretory stage of tooth enamel development. Thought to play a major role in the structural organization and mineralization of developing enamel. Defects in AMELX are the cause of amelogenesis imperfecta type 1E (AI1E). A X-linked defect of dental enamel formation. Teeth have only a thin layer of enamel with normal hardness. The thinness of the enamel makes the teeth appear small. Belongs to the amelogenin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: Xp22.31-p22.1
Cellular Component: cell surface; proteinaceous extracellular matrix
Molecular Function: growth factor activity; hydroxyapatite binding; identical protein binding; protein binding; structural constituent of tooth enamel
Biological Process: biomineral formation; cell adhesion; cell proliferation; chondrocyte differentiation; epithelial to mesenchymal transition; ion homeostasis; odontogenesis of dentine-containing teeth; osteoblast differentiation; positive regulation of collagen biosynthetic process; signal transduction
Disease: Amelogenesis Imperfecta, Type Ie
Product Notes
The Mouse Amelx amelx (Catalog #
AAA765682) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765682 ELISA Kit recognizes Mouse Amelx.
It is sometimes possible for the material contained within the vial of
"Amelogenin, X isoform, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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