Human Arachidonate 15-lipoxygenase B ELISA Kit | ALOX15B elisa kit

Human Arachidonate 15-lipoxygenase B ELISA Kit

Cat. Num. AAA760926

• Reactivity: Human
• Synonyms: Arachidonate 15-lipoxygenase B; Human Arachidonate 15-lipoxygenase B ELISA Kit; ALOX15B (Arachidonate 15-lipoxygenase B)/15-LOX-B/15-LOX-2/Linoleate 13-lipoxygenase 15-Lob/15-lipoxygenase 2/Arachidonate 15-lipoxygenase type II; ALOX15B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ALOX15B. No significant cross-reactivity or interference between ALOX15B and analogues was observed.
• Sequence Length: 677

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALOX15B elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 23463277
• NCBI GeneID: 266604
• NCBI Accession #: NP_695213.1
• NCBI GenBank Nucleotide #: NM_153301.2
• Molecular Weight: 76,145 Da
• NCBI Official Full Name: arachidonate 15-lipoxygenase B
• NCBI Official Synonym Full Names: arachidonate 15-lipoxygenase, type B
• NCBI Official Symbol: Alox15b
• NCBI Protein Information: arachidonate 15-lipoxygenase B
• UniProt Protein Name: Arachidonate 15-lipoxygenase B
• Protein Family: Arachidonate 15-lipoxygenase
• UniProt Gene Name: Alox15b
• UniProt Synonym Gene Names: 15-LOX-B; 15-LOX-2
• UniProt Entry Name: LX15B_RAT

NCBI Description

human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]

Uniprot Description

ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; Oxidoreductase; Lipid Metabolism - arachidonic acid; EC 1.13.11.33; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; cytoskeleton; cytosol; extrinsic to membrane; intracellular; membrane; nucleus; plasma membrane

Molecular Function: arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipid binding; lipoxygenase activity

Biological Process: arachidonic acid metabolic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of growth; positive regulation of keratinocyte differentiation