Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Arachidonate-15-Lipoxygenase Polyclonal Antibody | anti-ALOX15B antibody
Polyclonal Antibody to Arachidonate-15-Lipoxygenase, Type B (ALOX15B)
Immunohistochemistry: 5-20ug/mL;1:35-140
Immunocytochemistry: 5-20ug/mL;1:35-140
Optimal working dilutions must be determined by end user.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded Kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Kidney Tissue))
NCBI and Uniprot Product Information
NCBI Description
human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]
Uniprot Description
ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Cell cycle regulation; EC 1.13.11.33; Lipid Metabolism - arachidonic acid; Motility/polarity/chemotaxis; Oxidoreductase
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: cytoskeleton; cytosol; extrinsic component of membrane; intracellular; membrane; nucleus; plasma membrane
Molecular Function: arachidonate 15-lipoxygenase activity; calcium ion binding; iron ion binding; lipid binding; lipoxygenase activity
Biological Process: arachidonic acid metabolic process; hepoxilin biosynthetic process; linoleic acid metabolic process; lipoxygenase pathway; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of growth; positive regulation of keratinocyte differentiation
Research Articles on ALOX15B
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Product Notes
The ALOX15B alox15b (Catalog #AAA2027616) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Arachidonate-15-Lipoxygenase, Type B (ALOX15B) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Arachidonate-15-Lipoxygenase can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2ug/mL;1:350-1400 Immunohistochemistry: 5-20ug/mL;1:35-140 Immunocytochemistry: 5-20ug/mL;1:35-140 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ALOX15B alox15b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arachidonate-15-Lipoxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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