Rat 4-trimethylaminobutyraldehyde dehydrogenase ELISA Kit | ALDH9A1 elisa kit

Rat 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) ELISA Kit

Cat. Num. AAA285817

• Gene Names: Aldh9a1; Tmaba-dh
• Reactivity: Rat
• Synonyms: 4-trimethylaminobutyraldehyde dehydrogenase; Rat 4-trimethylaminobutyraldehyde dehydrogenase (ALDH9A1) ELISA Kit; ALDH4; ALDH7; ALDH9; E3; TMABADH; 4-trimethylaminobutyraldehyde dehydrogenase|R-aminobutyraldehyde dehydrogenase|aldehyde dehydrogenase (NAD+)|aldehyde dehydrogenase 9A1|aldehyde dehydrogenase E3 is; ALDH9A1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat ALDH9A1. No significant cross-reactivity or interference between Rat ALDH9A1 and analogues was observed.
• Sequence Length: 518

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ALDH9A1 elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ALDH9A1 in samples. An antibody specific for ALDH9A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyALDH9A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALDH9A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALDH9A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 75905479
• NCBI GeneID: 64040
• NCBI Accession #: NP_071609.2
• NCBI GenBank Nucleotide #: NM_022273.2
• UniProt Accession #: Q9JLJ3; Q6GMM4
• Molecular Weight: 53,653 Da
• NCBI Official Full Name: 4-trimethylaminobutyraldehyde dehydrogenase
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 9 family, member A1
• NCBI Official Symbol: Aldh9a1
• NCBI Official Synonym Symbols: Tmaba-dh
• NCBI Protein Information: 4-trimethylaminobutyraldehyde dehydrogenase
• UniProt Protein Name: 4-trimethylaminobutyraldehyde dehydrogenase
• Protein Family: 4-trimethylaminobutyraldehyde dehydrogenase
• UniProt Gene Name: Aldh9a1
• UniProt Synonym Gene Names: TMABADH

NCBI Description

catalyzes the conversion of gamma-trimethylaminobutyraldehyde to gamma-butyrobetaine in carnitine biosynthesis [RGD, Feb 2006]

Uniprot Description

ALDH9A1: Converts gamma-trimethylaminobutyraldehyde into gamma- butyrobetaine. Catalyzes the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction. Belongs to the aldehyde dehydrogenase family.

Protein type: Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - histidine; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Carbohydrate Metabolism - pyruvate; EC 1.2.1.3; EC 1.2.1.47; Lipid Metabolism - fatty acid; Lipid Metabolism - glycerolipid; Mitochondrial; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase; Secondary Metabolites Metabolism - limonene and pinene degradation

Chromosomal Location of Human Ortholog: 13q24

Cellular Component: cytosol; mitochondrion

Molecular Function: 3-chloroallyl aldehyde dehydrogenase activity; 4-trimethylammoniobutyraldehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; amine binding; aminobutyraldehyde dehydrogenase activity; NAD binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; protein homodimerization activity

Biological Process: aldehyde metabolic process; carnitine biosynthetic process; carnitine metabolic process; kidney development; liver development; neurotransmitter biosynthetic process