ALDH3A1 recombinant protein

Recombinant Human ALDH3A1 Protein (His tag)

Cat. Num. AAA2545491

• Gene Names: ALDH3A1; ALDH3; ALDHIII
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: ALDH3A1; Recombinant Human ALDH3A1 Protein (His tag); ALDH3; ALDHIII; MGC10406; ALDH3A1 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly
• Sequence Length: 453

• Application Notes: The recombinant human ALDH3A1 consists of 472 amino acids and has a calculated molecular mass of 52.6 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ALDH3A1 recombinant protein

A DNA sequence encoding the human ALDH3A1 (AAH04370.1) (Met 1-His 453) was fused with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 13325114
• NCBI GeneID: 218
• Molecular Weight: 50,395 Da
• NCBI Official Full Name: Aldehyde dehydrogenase 3 family, memberA1
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 3 family member A1
• NCBI Official Symbol: ALDH3A1
• NCBI Official Synonym Symbols: ALDH3; ALDHIII
• NCBI Protein Information: aldehyde dehydrogenase, dimeric NADP-preferring
• UniProt Protein Name: Aldehyde dehydrogenase, dimeric NADP-preferring
• Protein Family: Aldehyde dehydrogenase
• UniProt Gene Name: ALDH3A1
• UniProt Synonym Gene Names: ALDH3

NCBI Description

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]

Uniprot Description

ALDH3A1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. Belongs to the aldehyde dehydrogenase family.

Protein type: Amino Acid Metabolism - histidine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.5; Oxidoreductase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; extracellular space; intracellular membrane-bound organelle; nucleoplasm; plasma membrane

Molecular Function: 3-chloroallyl aldehyde dehydrogenase activity; alcohol dehydrogenase (NADP+) activity; aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; benzaldehyde dehydrogenase (NAD+) activity; protein binding

Biological Process: aldehyde metabolic process; xenobiotic metabolic process