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Western Blot (WB) (Western blot analysis of WNT9A using K562 whole cell lysates)

Rabbit anti-Human, Mouse WNT9A Polyclonal Antibody | anti-WNT9A antibody

WNT9A Antibody

Gene Names
WNT9A; WNT14
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
WNT9A; Polyclonal Antibody; WNT9A Antibody; MGC138165; MGC141991; Protein Wnt-14; Protein Wnt-9a; RP23-378H5.1; Wingless related MMTV integration site 9A; Wingless type MMTV integration site 9A; Wingless type MMTV integration site family; member 9A; wingless-type MMTV integration site family; member 14; Wnt 14; Wnt 9a; WNT14; WNT9A_HUMAN; anti-WNT9A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
WNT9A antibody detects endogenous levels of total WNT9A
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
365
Applicable Applications for anti-WNT9A antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human WNT9A
Subcellular Location
Secreted > Extracellular Space > Extracellular Matrix.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of WNT9A using K562 whole cell lysates)

Western Blot (WB) (Western blot analysis of WNT9A using K562 whole cell lysates)
Related Product Information for anti-WNT9A antibody
Function: Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.
Subunit Structure: Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity (PubMed:26902720). The complex with AFM may represent the physiological form in body fluids (PubMed:26902720).
Post-translational Modifications: Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.
Similarity: Belongs to the Wnt family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 40 kDa
Predicted: 41 kDa
NCBI Official Full Name
protein Wnt-9a
NCBI Official Synonym Full Names
Wnt family member 9A
NCBI Official Symbol
WNT9A
NCBI Official Synonym Symbols
WNT14
NCBI Protein Information
protein Wnt-9a
UniProt Protein Name
Protein Wnt-9a
Protein Family
UniProt Gene Name
WNT9A
UniProt Synonym Gene Names
WNT14

NCBI Description

The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is expressed in gastric cancer cell lines. The protein encoded by this gene shows 75% amino acid identity to chicken Wnt14, which has been shown to play a central role in initiating synovial joint formation in the chick limb. This gene is clustered with another family member, WNT3A, in the chromosome 1q42 region. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.

Research Articles on WNT9A

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Product Notes

The WNT9A wnt9a (Catalog #AAA9609361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The WNT9A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNT9A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the WNT9A wnt9a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT9A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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