Typical Testing Data/Standard Curve (for reference only)
Rat Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1) ELISA Kit | ALDH3A1 elisa kit
Rat Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ALDH3A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALDH3A1 antibody and an ALDH3A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH3A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH3A1 concentration since ALDH3A1 from samples and ALDH3A1-HRP conjugate compete for the anti-ALDH3A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH3A1 from the sample, fewer sites are left to bind ALDH3A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH3A1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]
Uniprot Description
ALDH3A1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. Belongs to the aldehyde dehydrogenase family.
Protein type: Amino Acid Metabolism - histidine; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.2.1.5; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: nucleoplasm; extracellular space; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; cytosol
Molecular Function: aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; alcohol dehydrogenase (NADP+) activity
Biological Process: response to drug; response to cAMP; positive regulation of cell proliferation; response to glucocorticoid stimulus; response to hypoxia; aldehyde metabolic process; response to nutrient; aging
Research Articles on ALDH3A1
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Product Notes
The Rat ALDH3A1 aldh3a1 (Catalog #AAA7206204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7206204 ELISA Kit recognizes Rat ALDH3A1. It is sometimes possible for the material contained within the vial of "Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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