Typical Testing Data/Standard Curve (for reference only)
Rabbit Aldehyde dehydrogenase family 1 member A3 ELISA Kit | ALDH1A3 elisa kit
Rabbit Aldehyde dehydrogenase family 1 member A3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
INTENDED USE This APN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit APN. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY APN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APN antibody and an APN-HRP conjugate. The assay sample and buffer are incubated together with APN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APN concentration since APN from samples and APN-HRP conjugate compete for the anti-APN antibody binding site. Since the number of sites is limited, as more sites are occupied by APN from the sample, fewer sites are left to bind APN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APN concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]
Uniprot Description
ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family.
Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.2.1.5; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - histidine; Amino Acid Metabolism - phenylalanine; Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Chromosomal Location of Human Ortholog: 15q26.3
Cellular Component: cytoplasm; cytosol
Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity
Biological Process: inner ear morphogenesis; retinal metabolic process; retinol metabolic process; positive regulation of apoptosis; embryonic eye morphogenesis; righting reflex; olfactory pit development; retinoic acid metabolic process; optic cup morphogenesis involved in camera-type eye development; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development
Disease: Microphthalmia, Isolated 8
Research Articles on ALDH1A3
Similar Products
Product Notes
The Rabbit ALDH1A3 aldh1a3 (Catalog #AAA730865) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA730865 ELISA Kit recognizes Rabbit ALDH1A3. It is sometimes possible for the material contained within the vial of "Aldehyde dehydrogenase family 1 member A3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
