ALDH1A3 recombinant protein

Recombinant Human ALDH1A3

Cat. Num. AAA7614650

• Gene Names: ALDH1A3; ALDH6; MCOP8; RALDH3; ALDH1A6
• Applications: Western Blot, ELISA
• Purity: Greater than 50% by SDS-PAGE gel analyses
• Synonyms: ALDH1A3; Recombinant Human ALDH1A3; Aldehyde dehydrogenase 6; ALDH1A6; ALDH6; RALDH 3; RALDH3; Retinaldehyde dehydrogenase 3; ALDH1A3 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 50% by SDS-PAGE gel analyses
• Form/Format: Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose and 0.01% proclin, pH7.4
• Sequence Positions: 1-180

• Applicable Applications for ALDH1A3 recombinant protein: Western Blot (WB), ELISA (EIA)
• Species: Human
• Tag: N-terminal His-IF2DI Tag
• Reconstitution: Centrifuge the vial at 10,000rpm for 1 minute, reconstitute at 200ug/ml in sterile distilled water
• Preparation and Storage: Store at -20 degree C for 12 months as lyophilized; 2-8 degree C for 1 month under sterile conditions after reconstitution

NCBI and Uniprot Product Information

• NCBI GI #: 52788258
• NCBI GeneID: 220
• UniProt Accession #: P47895; Q6NT64
• Molecular Weight: 512
• NCBI Official Full Name: Aldehyde dehydrogenase family 1 member A3
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 1 family, member A3
• NCBI Official Symbol: ALDH1A3
• NCBI Official Synonym Symbols: ALDH6; MCOP8; RALDH3; ALDH1A6
• NCBI Protein Information: aldehyde dehydrogenase family 1 member A3; aldehyde dehydrogenase 6; acetaldehyde dehydrogenase 6; retinaldehyde dehydrogenase 3
• UniProt Protein Name: Aldehyde dehydrogenase family 1 member A3
• Protein Family: Aldehyde dehydrogenase family
• UniProt Gene Name: ALDH1A3
• UniProt Synonym Gene Names: ALDH6; RALDH-3; RalDH3
• UniProt Entry Name: AL1A3_HUMAN

NCBI Description

This gene encodes an aldehyde dehydrogenase enzyme that uses retinal as a substrate. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

ALDH1A3: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Seems to be the key enzyme in the formation of an RA gradient along the dorso-ventral axis during the early eye development and also in the development of the olfactory system. Belongs to the aldehyde dehydrogenase family.

Protein type: Oxidoreductase; Amino Acid Metabolism - histidine; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.2.1.5; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; protein homodimerization activity; aldehyde dehydrogenase [NAD(P)+] activity; retinal dehydrogenase activity

Biological Process: inner ear morphogenesis; retinal metabolic process; righting reflex; embryonic eye morphogenesis; retinol metabolic process; positive regulation of apoptosis; olfactory pit development; retinoic acid metabolic process; optic cup morphogenesis involved in camera-type eye development; locomotory behavior; neuromuscular process controlling balance; nucleus accumbens development

Disease: Microphthalmia, Isolated 8