Rat Alpha-2-HS-glycoprotein ELISA Kit | Ahsg elisa kit

Rat Alpha-2-HS-glycoprotein ELISA Kit

Cat. Num. AAA764331

• Reactivity: Rat
• Synonyms: Alpha-2-HS-glycoprotein; Rat Alpha-2-HS-glycoprotein ELISA Kit; Ahsg/AHSG/alpha-2-HS-glycoprotein/A2HS/AHS/alpha-2-HS-glycoprotein/alpha-2-Z-globulin/Alpha-2-Z-globulin/Ba-alpha-2-glycoprotein/FETUAba-alpha-2-glycoprotein/fetuin-A/Fetuin A/HSGA; Ahsg elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Ahsg. No significant cross-reactivity or interference between Ahsg and analogues was observed.
• Sequence Length: 367

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 78-5000pg/ml
• Sensitivity: <46.9pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ahsg were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ahsg were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Ahsg elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Ahsg antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Ahsg antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Ahsg amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Ahsg can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 178284
• Molecular Weight: 39,325 Da
• NCBI Official Full Name: alpha-2-HS-glycoprotein
• UniProt Protein Name: Alpha-2-HS-glycoprotein
• Protein Family: Alpha-2-HS-glycoprotein
• UniProt Gene Name: AHSG
• UniProt Synonym Gene Names: FETUA
• UniProt Entry Name: FETUA_HUMAN

Uniprot Description

FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular matrix; extracellular region; extracellular space

Molecular Function: cysteine protease inhibitor activity; endopeptidase inhibitor activity; kinase inhibitor activity

Biological Process: acute-phase response; negative regulation of bone mineralization; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; ossification; pinocytosis; positive regulation of phagocytosis; regulation of bone mineralization; regulation of inflammatory response; skeletal development