ADK recombinant protein

ADK, 22-362aa, Human, His tag, E Coli

Cat. Num. AAA203486

• Gene Names: ADK; AK
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: ADK; 22-362aa; Human; His tag; E Coli; AK; adenosine kinase; adenosine 5'-phosphotransferase; ; ADK recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH
• Sequence Length: 362

• Applicable Applications for ADK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ADK recombinant protein

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ADK recombinant protein

Cancer

References

Chakraborty, A., et al. (2002) J. Biol. Chem. 277:47451-47451.; Sakowicz M., et al. (2001) Acta Biochim Pol. 48 (3): 745-54.;

NCBI and Uniprot Product Information

• NCBI GI #: 32484975
• NCBI GeneID: 132
• NCBI Accession #: NP_006712
• NCBI GenBank Nucleotide #: NM_006721.3
• UniProt Accession #: P55263; O00741; O00742; Q16710; Q5JQ10; Q5JQ11; Q9BTN2; B7Z783; B7Z800
• Molecular Weight: 40.5 kDa (362aa), confirmed by MALDI-TOF
• NCBI Official Full Name: adenosine kinase isoform b
• NCBI Official Synonym Full Names: adenosine kinase
• NCBI Official Symbol: ADK
• NCBI Official Synonym Symbols: AK
• NCBI Protein Information: adenosine kinase
• UniProt Protein Name: Adenosine kinase
• UniProt Gene Name: ADK
• UniProt Synonym Gene Names: AK

NCBI Description

This gene an enzyme which catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. Serves as a potential regulator of concentrations of extracellular adenosine and intracellular adenine nucleotides.