SFRP3 Active Protein | FRZB active protein

SFRP3, Recombinant, Human (Secreted Frizzled Related Protein 3, FRZB, Fritz, Frzb1) (BSA Free)

Cat. Num. AAA636596

• Gene Names: FRZB; FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
• Purity: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: SFRP3; Recombinant; Human (Secreted Frizzled Related Protein 3; FRZB; Fritz; Frzb1) (BSA Free); FRZB active protein

• Purity/Purification: Highly Purified; 95%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS, pH 7.2.

• Activity: Measured by its ability to interfere with the axis inducing activity of Xwnt-8 mRNA in early Xenopus embryos. In a population of embryos, addition of 1ng of rhsFRP protein per embryo resulted in 50-100% reduction in number of secondary axes produced by 10-20pg of Xwnt-8 mRNA.
• Endotoxin Level: 1.0 EU per 1ug of the cytokine as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 50ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for greater than six months at -20 degree C to -70 degree C. Upon reconstitution, this cytokine, in the presence of carrier protein, can be stored under sterile conditions at 2 degree - 4 degree C for one month or at -20 degree C to -70 degree C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for FRZB active protein

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. sFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas. The N-terminal portion of the protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. sFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA (Hoang et al., 1996, J. Biol. Chem. 271:26131; Leyns et al., 1997, Cell 88:747; Wang et al.,1997, Cell 88:757; Mayr et al., 1997, Mech. Dev. 63:109; Rattner et al., 1997, PNAS 94:2859).

Product Categories/Family for FRZB active protein

Molecular Biology; MB-Wnt Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 38455388
• NCBI GeneID: 2487
• NCBI Accession #: NP_001454.2
• NCBI GenBank Nucleotide #: NM_001463.3
• UniProt Accession #: Q92765; O00181; Q99686
• Molecular Weight: Based on N-terminal sequencing, the mature human sFRP-3 protein starts at Ala33 and has a; calculated molecular mass of 35kD. As a result of glycosylation, the recombinant protein migrates as an ~38kD protein in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: secreted frizzled-related protein 3
• NCBI Official Synonym Full Names: frizzled-related protein
• NCBI Official Symbol: FRZB
• NCBI Official Synonym Symbols: FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
• NCBI Protein Information: secreted frizzled-related protein 3; sFRP-3; frezzled; frizzled homolog-related; frizzled-related protein 1
• UniProt Protein Name: Secreted frizzled-related protein 3
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: FRZB
• UniProt Synonym Gene Names: FIZ; FRE; FRP; FRZB1; SFRP3; sFRP-3
• UniProt Entry Name: SFRP3_HUMAN

NCBI Description

The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]

Uniprot Description

FRZB: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development. Defects in FRZB are associated with susceptibility to osteoarthritis type 1(OS1). Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity

Biological Process: negative regulation of cell proliferation; convergent extension involved in organogenesis; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of fat cell differentiation; negative regulation of cell growth; neural crest cell differentiation; skeletal development

Disease: Osteoarthritis Susceptibility 1