FRZB recombinant protein

FRZB Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120232

• Gene Names: FRZB; FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
• Purity: >85% as determined by SDS-PAGE
• Synonyms: FRZB; FRZB Protein; Human; Recombinant (His Tag); Human FRZB/sFRP-3 Protein (His Tag); FRE Protein; FRITZ Protein; FRP-3 Protein; FRZB-1 Protein; FRZB-PEN Protein; FRZB1 Protein; FZRB Protein; hFIZ Protein; OS1 Protein; SFRP3 Protein; SRFP3 Protein; frizzled-related protein; FRZB recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala32-Asn325

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ala 32
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human FRZB (NP_001454.2)(Ala32-Asn325) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FRZB recombinant protein

Background: FRZB also known as sFRP-3, is a secreted protein containing a domain similar to the putative Wnt-binding region of the frizzled family of transmembrane receptors. FRZB is widely expressed in adult mammalian tissues. In the Xenopus gastrula, FRZB is regulated as a typical Spemann organizer component. FRZB also functions as a competitor for the cell-surface G-protein receptor Frizzled. It is espically important in embryonic development. Defects in FRZB gene can cause female-specific osteoarthritis (OA) susceptibility. FRZB may serve an important role in determining hip shape and may modify the relationship between hip shape and OA.

References

Hoang B., et al.,(1996), Primary structure and tissue distribution of FRZB, a novel protein related to Drosophila frizzled, suggest a role in skeletal morphogenesis. J. Biol. Chem. 271:26131-26137.Mayr T., et al., (1997), Fritz: a secreted frizzled-related protein that inhibits Wnt activity.Mech. Dev. 63:109-125.Leyns L., et al.,(1997), Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer.Cell 88:747-756.

NCBI and Uniprot Product Information

• NCBI GI #: 38455388
• NCBI GeneID: 2487
• NCBI Accession #: NP_001454.2
• NCBI GenBank Nucleotide #: NM_001463.3
• UniProt Accession #: Q92765; O00181; Q99686
• Molecular Weight: 36,254 Da
• NCBI Official Full Name: secreted frizzled-related protein 3
• NCBI Official Synonym Full Names: frizzled-related protein
• NCBI Official Symbol: FRZB
• NCBI Official Synonym Symbols: FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
• NCBI Protein Information: secreted frizzled-related protein 3; frezzled; frizzled homolog-related; frizzled-related protein 1; sFRP-3
• UniProt Protein Name: Secreted frizzled-related protein 3
• Protein Family: Frizzy aggregation protein
• UniProt Gene Name: FRZB
• UniProt Synonym Gene Names: FIZ; FRE; FRP; FRZB1; SFRP3; sFRP-3
• UniProt Entry Name: SFRP3_HUMAN

NCBI Description

The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]

Uniprot Description

FRZB: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development. Defects in FRZB are associated with susceptibility to osteoarthritis type 1(OS1). Osteoarthritis is a degenerative disease of the joints characterized by degradation of the hyaline articular cartilage and remodeling of the subchondral bone with sclerosis. Clinical symptoms include pain and joint stiffness often leading to significant disability and joint replacement. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q32.1

Cellular Component: extracellular space; membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity

Biological Process: negative regulation of cell proliferation; convergent extension involved in organogenesis; negative regulation of Wnt receptor signaling pathway; positive regulation of apoptosis; positive regulation of fat cell differentiation; neural crest cell differentiation; negative regulation of cell growth; skeletal development

Disease: Osteoarthritis Susceptibility 1