Typical Testing Data/Standard Curve (for reference only)
Human Secreted Frizzled Related Protein 3 (SFRP3) ELISA Kit | SFRP3 elisa kit
Human Secreted Frizzled Related Protein 3 (SFRP3) ELISA Kit
Gene Names
FRZB; FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
Reactivity
Human
Synonyms
Secreted Frizzled Related Protein 3 (SFRP3); Human Secreted Frizzled Related Protein 3 (SFRP3) ELISA Kit; SFRP3 elisa kit
Reactivity
Human
Sequence Length
325
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml - 30ng/ml
Sensitivity
0.021ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SFRP3 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Secreted Frizzled Related Protein 3 (also known as SFRP3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SFRP3 antibody. SFRP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SFRP3 Antibody is added and binds to SFRP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFRP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SFRP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human SFRP3 antibody. SFRP3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human SFRP3 Antibody is added and binds to SFRP3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SFRP3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human SFRP3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36,254 Da
NCBI Official Full Name
secreted frizzled-related protein 3
NCBI Official Synonym Full Names
frizzled related protein
NCBI Official Symbol
FRZB
NCBI Official Synonym Symbols
FRE; OS1; FZRB; hFIZ; FRITZ; FRP-3; FRZB1; SFRP3; SRFP3; FRZB-1; FRZB-PEN
NCBI Protein Information
secreted frizzled-related protein 3
UniProt Protein Name
Secreted frizzled-related protein 3
UniProt Gene Name
FRZB
UniProt Synonym Gene Names
FIZ; FRE; FRP; FRZB1; SFRP3; sFRP-3
NCBI Description
The protein encoded by this gene is a secreted protein that is involved in the regulation of bone development. Defects in this gene are a cause of female-specific osteoarthritis (OA) susceptibility. [provided by RefSeq, Apr 2010]
Uniprot Description
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development.
Research Articles on SFRP3
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Product Notes
The Human SFRP3 frzb (Catalog #AAA167239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA167239 ELISA Kit recognizes Human SFRP3. It is sometimes possible for the material contained within the vial of "Secreted Frizzled Related Protein 3 (SFRP3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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