GRO Beta/CXCL2 Recombinant Protein | CXCL2 recombinant protein

GRO Beta/CXCL2 Protein, Human, Recombinant

Cat. Num. AAA8120372

• Gene Names: CXCL2; GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
• Purity: >95% as determined by SDS-PAGE
• Synonyms: GRO Beta/CXCL2; GRO Beta/CXCL2 Protein; Human; Recombinant; Human CXCL2/MIP-2 Protein; CINC-2a Protein; GRO2 Protein; GROb Protein; MGSA-b Protein; MIP-2a Protein; MIP2 Protein; MIP2A Protein; SCYB2 Protein; chemokine (C-X-C motif) ligand 2; CXCL2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 1 M NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Ala35-Asn107

• Species: Human
• Predicted N Terminal: Ala 35
• Tag: N-cleavage
• Protein Construction: A DNA sequence encoding the human CXCL2 (NP_002080.1)(Ala35-Asn107) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CXCL2 recombinant protein

Background: Chemokine (C-X-C motif) ligand 2 (CXCL2), also called macrophage inflammatory protein 2 (MIP-2), Growth-regulated protein beta (Gro-beta) and Gro oncogene-2 (Gro-2), is a small cytokine belonging to the CXC chemokine family. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone, where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for CXCL2/MIP-2 or in the presence of a blocking Ab to CXCL2/MIP-2, peripheral tolerance is prevented, and Ag-specific T regulatory cells are not generated. CXCL2/MIP-2 is selectively up-regulated in tolerance-conferring APCs and serves to recruit NKT cells to the splenic marginal zone, where they form clusters with APCs and T cells. In the absence of the high-affinity receptor for MIP-2 (as in CXCR2-deficient mice) or in the presence of a blocking Ab to MIP-2, peripheral tolerance is prevented, and Ag-specific T regulatory cells are not generated. Understanding the regulation of lymphocyte traffic during tolerance induction may lead to novel therapies for autoimmunity, graft acceptance, and tumor rejection. Several studies have implicated the CXCL2 chemokine as a mediator in the development of sepsis. CXCL2/MIP-2 also plays a major role in mediating the neutrophilic inflammatory response of the rodent lung to particles such as quartz, crocidolite asbestos, as well as high doses of other relative innocuous dusts such as titanium dioxide.

Product Categories/Family for CXCL2 recombinant protein

Neuroinflammation; IL17 signaling pathway

References

Driscoll KE. (2000) TNFalpha and MIP-2: role in particle-induced inflammation and regulation by oxidative stress. Toxicol Lett. 112-113: 177-83.Walpen S, et al. (2001) Nitric oxide induces MIP-2 transcription in rat renal mesangial cells and in a rat model of glomerulonephritis. FASEB J. 15(3): 571-3.Fahey TJ, et al. (1990) Cytokine production in a model of wound healing: the appearance of MIP-1, MIP-2, cachectin/TNF and IL-1. Cytokine. 2(2): 92-9.

NCBI and Uniprot Product Information

• NCBI GI #: 4504155
• NCBI GeneID: 2920
• NCBI Accession #: NP_002080.1
• NCBI GenBank Nucleotide #: NM_002089.3
• UniProt Accession #: P19875; Q6FGD6; Q9UPB8
• Molecular Weight: 11,389 Da
• NCBI Official Full Name: C-X-C motif chemokine 2
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 2
• NCBI Official Symbol: CXCL2
• NCBI Official Synonym Symbols: GRO2; GROb; MIP2; MIP2A; SCYB2; MGSA-b; MIP-2a; CINC-2a
• NCBI Protein Information: C-X-C motif chemokine 2; GRO2 oncogene; MGSA beta; MIP2-alpha; gro-beta; growth-regulated protein beta; macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta
• UniProt Protein Name: C-X-C motif chemokine 2
• Protein Family: C-X-C motif chemokine
• UniProt Gene Name: CXCL2
• UniProt Synonym Gene Names: GRO2; GROB; MIP2A; SCYB2; Gro-beta; MIP2-alpha; HSF
• UniProt Entry Name: CXCL2_HUMAN

NCBI Description

This antimicrobial gene is part of a chemokine superfamily that encodes secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CXC subfamily, is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. [provided by RefSeq, Sep 2014]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity; CXCR chemokine receptor binding

Biological Process: G-protein coupled receptor protein signaling pathway; response to molecule of bacterial origin; response to lipopolysaccharide; immune response; positive regulation of leukocyte chemotaxis; chemotaxis; inflammatory response; regulation of cell proliferation