Testing Data
(specific activity)
RAF Active Protein
RAF, Recombinant, Human (A Raf, A-raf-1, ARaf Proto-oncogene Serine/threonine Protein Kinase, ARAF1, ARAF, Oncogene PKS2, PKS, PKS2, Proto-oncogene Pks, RAFA1, Ras-binding Protein DA-Raf, v-Raf Mouse Sarcoma 3611 Viral Oncogene Homolog)
Purity
Purified0.8
Synonyms
RAF; Recombinant; Human (A Raf; A-raf-1; ARaf Proto-oncogene Serine/threonine Protein Kinase; ARAF1; ARAF; Oncogene PKS2; PKS; PKS2; Proto-oncogene Pks; RAFA1; Ras-binding Protein DA-Raf; v-Raf Mouse Sarcoma 3611 Viral Oncogene Homolog); RAF active protein
Purity/Purification
Purified
0.8
0.8
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween-20, 50% glycerol, 3mM DTT.
Application Notes
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Specific Activity
50nM aRAF yields 8% conversion of 1uM ATP using MEK1 (K97R) as substrate.
Reaction Buffer:
10mM HEPES (pH7.4), 5mM MgCl2, 10uM MnCl2, 1mM DTT, 3uM Naorthovanadate, 1uM ATP.
Reaction Buffer:
10mM HEPES (pH7.4), 5mM MgCl2, 10uM MnCl2, 1mM DTT, 3uM Naorthovanadate, 1uM ATP.
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Testing Data
(specific activity)
Testing Data
(specific activity)
SDS-Page
(10% SDS-PAGE Coomassie staining. |Lane 1: 2ug R0495-05A|Lane 2: MW marker|MW: 63kD |Purity: ≥62%)
SDS-Page
(10% SDS-PAGE Coomassie staining. |Lane 1: 2ug R0495-05A|Lane 2: MW marker|MW: 63kD |Purity: ≥62%)
Related Product Information for RAF active protein
The RAF family of signaling proteins has three isoforms: A RAF, RAF 1 and B RAF. These proteins are instrumental in forwarding the stimulus generated by mitogens from monomeric G proteins to the MAP kinase pathway. RAF 1 is known to be downstream of Ras and binds to it by an N terminal noncatalytic domain. This domain is conserved in A RAF and B RAF. 14-3-3 is also known to be an activator of RAFs. Activated RAF directly phosphorylates and activates MEK. A RAF is expressed at a very high concentration in urogenital and kidney tissues.
Product Categories/Family for RAF active protein
NCBI and Uniprot Product Information
Uniprot Description
Antagonizes transcriptional repression of recombinase FLP by REP1-REP2. May play a role in plasmid partitioning.
Similar Products
Product Notes
The RAF (Catalog #AAA637418) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Researchers should empirically determine the suitability of the RAF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAF, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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