Western Blot (WB)
(Western Blot (WB) analysis of CD30 (H559) polyclonal antibody at 1:1000 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Rat (40ug))
Rabbit CD30 Polyclonal Antibody | anti-CD30 antibody
CD30 (H559) Polyclonal Antibody
IHC: 1:50-1:100
IP: 1:10-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of CD30 (H559) polyclonal antibody at 1:1000 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Rat (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of CD30 (H559) polyclonal antibody at 1:1000 dilutionLane1:HCT116 whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:CT26 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug) Lane5:The Testis tissue lysate of Rat (40ug))
Immunoprecipitation (IP)
(Immunoprecipitation of the Testis tissue lysate of Rat using CD30 (H559) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5). Lane 1 is 30% input. The Western Blot was probed using CD30 (H559) pAb # MBS3000247. “up-arrow” (supernatant) ; “down-arrow” (deposition))
Immunoprecipitation (IP)
(Immunoprecipitation of the Testis tissue lysate of Rat using CD30 (H559) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5). Lane 1 is 30% input. The Western Blot was probed using CD30 (H559) pAb # MBS3000247. “up-arrow” (supernatant) ; “down-arrow” (deposition))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Uniprot Description
TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1p36
Cellular Component: integral to plasma membrane
Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity
Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction
Research Articles on CD30
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Product Notes
The CD30 tnfrsf8 (Catalog #AAA3000247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CD30 (H559) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD30 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:100 IP: 1:10-1:100. Researchers should empirically determine the suitability of the CD30 tnfrsf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD30, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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