Mannan Binding Protein Active Protein | Mbl2 active protein

Mannan Binding Protein, Recombinant, Mouse (MBP, Mannose Binding Protein, Mannan Binding Lectin, MBL)

Cat. Num. AAA637558

• Gene Names: Mbl2; Ab2-001; Ab2-011
• Purity: Affinity Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
• Synonyms: Mannan Binding Protein; Recombinant; Mouse (MBP; Mannose Binding Protein; Mannan Binding Lectin; MBL); Mbl2 active protein

• Purity/Purification: Affinity Purified; 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1EU/ug (LAL)
• Form/Format: Supplied as a lyophilized powder from PBS, 50ug of BSA per 1ug of cytokine. Reconstitute with sterile PBS, 0.1% HSA or BSA to a concentration of 0.1mg/ml.

• Activity: Measured by its ability to bind with Mannan.
• Endotoxin Level: 1EU/ug of the cytokine as determined by the LAL method.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Mbl2 active protein

Mannan binding lectin (MBL) belongs to the collectin family of innate immune defense proteins, which binds to an array of carbohydrate patterns on pathogen surfaces.1, 2 Collectin family members share common structural features: a cysteine rich amino-terminal domain, a collagen-like region, an -helical coiled-coil neck domain and a carboxy terminal C-type

In mouse, MBL-1 and MBL-2 are the only collectins that can activate complement via the lectin complement pathway.1, 2 Serumoligomeric MBL associates with MBL-associated serine protease (MASP) proenzymes. The MBL-MASP proenzyme complex preferentially interact with sugar patterns containing mannose, glucose, L-fucose, or N-acetyl-glucosamine present at a terminal nonreducing postion on the cell surface of various pathogens and certain tumor cells. This interaction induces pro-enzyme activation and the triggering of the complement cascade, resulting in opsonization and pathogen removal via humoral and cellular immune responses. MBL does not recognize self-components or glycoproteins from other higher animals due to the presence ofterminal sialic acid or galactose that interrupts the repeating carbohydrate structures.3 A number of membrane receptors for MBL, including C1q phagocytic receptor (C1qRp), calreticulin (also known as C1qR), and CR1 (CD35), have been described. Interactions with these receptors may also be important in stimulating phagocytosis,1, 2 Mouse MBL-1 and MBL-2 are produced primarily in the liver and are secreted into the blood stream. In addition, mouse MBL-1 is also expressed in lung, kidney and testis while MBL-2 is expressed in kidney, thymus and small intestine.1, 4, 5

Product Categories/Family for Mbl2 active protein

Lectins; Lectin-Mannan Binding Lectin

NCBI and Uniprot Product Information

• NCBI GI #: 148747321
• NCBI GeneID: 64668
• NCBI Accession #: NP_073195.2
• NCBI GenBank Nucleotide #: NM_022704.2
• UniProt Accession #: P08661
• Molecular Weight: The mature recombinant mature mouse MBL-1, generated by proteolytic removal of the signal peptide, is an oligomeric protein. Based on N-terminal sequencing, the recombinant mouse MBL-1 begins with Ser 18 and has a calculated monomeric molecular mass of ap
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose-binding lectin (protein C) 2
• NCBI Official Symbol: Mbl2
• NCBI Official Synonym Symbols: Ab2-001; Ab2-011
• NCBI Protein Information: mannose-binding protein C; MBP-C; RARF/P28A; raRF p28A; mannan-binding protein; RA-reactive factor P28A subunit; mannose-binding protein C (liver); mannose binding lectin 2 (protein C); ra-reactive factor polysaccharide-binding component p28A
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: Mannose-binding protein
• UniProt Gene Name: Mbl2
• UniProt Synonym Gene Names: MBP-C; RaRF p28A
• UniProt Entry Name: MBL2_RAT

NCBI Description

a lectin that binds mannose and N-acetylglucosamine [RGD, Feb 2006]

Uniprot Description

Function: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages

By similarity.

Subunit structure: Oligomeric complex of 3 or more homotrimers. Interacts with MASP1 and MASP2

By similarity. Interacts with MEP1A and MEP1B and may inhibit their catalytic activity

By similarity.

Subcellular location: Secreted

By similarity Ref.4.

Sequence similarities: Contains 1 C-type lectin domain.Contains 1 collagen-like domain.