LAIR-1 Active Protein | LAIR1 active protein

LAIR-1, Recombinant, Mouse (Leukocyte-associated Ig-like Receptor-1)

Cat. Num. AAA635514

• Gene Names: LAIR1; CD305; LAIR-1
• Purity: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: LAIR-1; Recombinant; Mouse (Leukocyte-associated Ig-like Receptor-1); LAIR1 active protein

• Purity/Purification: Highly Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder in PBS.

• Activity: Measured by the ability of the immobilized protein to support the adhesion of HT-29 cells. When 5 x 10e4 cells/well are added to recombinant mouse LAIR-1-coated plates (50ug/ml with 100 L/well), approximately 30-60% will adhere after 10 minutes at 37 degree C. Optimal concentration depends on cell type as well as the application or research objectives.
• Endotoxin Level: < 1.0 EU per 1 g of the protein as determined by the LAL method.
• Reconstitution: It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
• Preparation and Storage: Lyophilized samples are stable for up to twelve months at -20 degree C. Upon reconstitution, this protein, in the presence of a carrier protein, can be stored under sterile conditions at 2 degree -8 degree C for one month or at -20 degree C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Related Product Information for LAIR1 active protein

Leukocyte-associated Ig-like receptor-1 (LAIR-1) is an inhibitory receptor of the Ig superfamily that is structurally related to inhibitory members of KIR and ILT/CD85 families (1-3). It is expressed on immune cells, including NK cells, T cells, B cells, monocytes, immature neutrophils, dendritic cells and most thymocytes (2-4). The 253 amino acid (aa) type I transmembrane (TM) protein contains a 21 aa signal sequence, a 124 aa extracellular domain (ECD), a 20 aa TM domain and a 98 aa cytoplasmic domain. The ECD includes one C2-type Ig-like domain and two potential N-linked glycosylation sites. Tyrosine phosphorylation of two cytoplasmic ITIM motifs results in recruitment of phosphatases and downregulation of signaling through activating receptors (2, 3, 5). Crosslinking of LAIR-1 inhibits processes such as B cell receptor-mediated activation, NK cell and T cell cytotoxicity and basophil degranulation (1-3). Four mouse LAIR-1 splice variants have been identified, but it is not known whether they are expressed as proteins (3). LAIR1b, which is the major alternate transcript, lacks most of the ECD. Of the minor transcripts, LAIR-1c lacks a signal sequence, and LAIR-1d and 1e lack a TM sequence. All mouse splice forms are identical in the last 90 aa of the cytoplasmic domain. LAIR-1 shows high-affinity binding of collagens that results in inhibition of degranulation in a basophilic leukemia cell line (6). Human and mouse LAIR-1 ECD share only 32% aa identity but, where studied, sites of expression and activity are similar (3-6). Mouse LAIR-1 ECD also shares 62%, 31% and 28% aa identity with rat, dog, and cow orthologs, respectively.

Product Categories/Family for LAIR1 active protein

Molecular Biology; MB-Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 11231177
• NCBI GeneID: 3903
• NCBI Accession #: NP_068352.1
• NCBI GenBank Nucleotide #: NM_021706.2
• UniProt Accession #: Q6GTX8
• Molecular Weight: The recombinant mouse LAIR-1, generated by proteolytic removal of the signal peptide region, is a monomeric protein. Based on N-terminal amino acid sequencing, the major sequence was blocked, suggesting that the majority of the recombinant protein in the
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform b
• NCBI Official Synonym Full Names: leukocyte-associated immunoglobulin-like receptor 1
• NCBI Official Symbol: LAIR1
• NCBI Official Synonym Symbols: CD305; LAIR-1
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1; hLAIR1; leukocyte-associated Ig-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• Protein Family: Leukocyte-associated immunoglobulin-like receptor
• UniProt Gene Name: LAIR1
• UniProt Synonym Gene Names: CD305; LAIR-1; hLAIR1
• UniProt Entry Name: LAIR1_HUMAN

NCBI Description

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. [provided by RefSeq, Jul 2008]

Uniprot Description

LAIR-1: an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region called the leukocyte receptor cluster, which contains at least 25 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: immune system process