Klotho beta Active Protein | KLB active protein

Klotho beta, Recombinant, Mouse (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)

Cat. Num. AAA635824

• Gene Names: KLB; BKL
• Purity: Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Klotho beta; Recombinant; Mouse (Beta-klotho; BKL; BetaKlotho; Klotho beta-like Protein); KLB active protein

• Specificity: Recognizes mouse Klotho beta. species sequence homology: Human 79%, bovine 79%, canine 80% and rat Klotho beta 67%.
• Purity/Purification: Purified; 90%, as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a liquid in PBS, 0.1 mM EDTA, 50% glycerol, pH 6.8.

• Immunogen: NS0-derived recombinant mouse Klotho.
• Biological Activity: Measured by its ability to stimulate proliferation of NIH 3T3 cells. The ED50 for this effect is typically 0.75-3ug/ml in the presence of 2ug/ml of rhFGF-21
• Endotoxin Level: 1EU/ug of the protein as determined by the LAL method.
• Hybridoma: A DNA sequence encoding the extracellular domain of mouse Klotho Beta was fused to a polyhistdine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(1ug/lane of Recombinant Mouse Klotho beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 135kD.)

Related Product Information for KLB active protein

Klotho b, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue.1 Like Klotho, Klotho b facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex.2 The Klotho b mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-a hydroxylase (Cyp7a1) and sterol 12-a hydroxylase (Cyp8b1).2-4 Klotho b is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake.2-3, 5 The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in b-glycosidases, it was initially unclear whether they were functional enzymes.1, 6 However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho b is also possible.7 The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho b, respectively. The low identity with rat reflects aa discordance within rodent ECD.

Product Categories/Family for KLB active protein

Molecular Biology; MB-Enzymes; Glycosidase

NCBI and Uniprot Product Information

• NCBI GI #: 119613331
• NCBI GeneID: 152831
• UniProt Accession #: Q86Z14; Q2M3K8
• Molecular Weight: 110.2kD. As a result of glycosylation, the recombinant mouse Klotho Beta migrates as an approximately 140kD protein in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: klotho beta
• NCBI Official Synonym Full Names: klotho beta
• NCBI Official Symbol: KLB
• NCBI Official Synonym Symbols: BKL
• NCBI Protein Information: beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein
• UniProt Protein Name: Beta-klotho
• Protein Family: Beta-klotho
• UniProt Gene Name: KLB
• UniProt Synonym Gene Names: BKL; BetaKlotho
• UniProt Entry Name: KLOTB_HUMAN

Uniprot Description

Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; fibroblast growth factor binding; hydrolase activity, hydrolyzing O-glycosyl compounds; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response