Typical Testing Data/Standard Curve (for reference only)
(Human Klotho beta PicoKine ELISA Kit standard curve )
Human Klotho beta ELISA Kit | KLB elisa kit
Human Klotho beta PicoKine ELISA Kit
Gene Names
KLB; BKL
Reactivity
Human
Synonyms
Klotho beta; Human Klotho beta PicoKine ELISA Kit; Beta-klotho; Betaklotho; BKL; KLB; Q86Z14; KLB elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human Klotho beta
Sequence Length
1044
Samples
Cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
31.2pg/ml-2000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Human Klotho beta PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Klotho beta PicoKine ELISA Kit standard curve )
Related Product Information for KLB elisa kit
Principle of the Assay: The Human Klotho beta ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for Klotho beta has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: F53-L997) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Klotho beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Klotho beta amount of sample captured in plate.
Background: Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. It is mapped to chromosome 4. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also an essential cofactor for FGF21 activity and the Klotho gene family may have evolved to confer tissue-specific bioactivity on FGF19 subfamily members.
Background: Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. It is mapped to chromosome 4. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also an essential cofactor for FGF21 activity and the Klotho gene family may have evolved to confer tissue-specific bioactivity on FGF19 subfamily members.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
119,808 Da
NCBI Official Full Name
beta-klotho
NCBI Official Synonym Full Names
klotho beta
NCBI Official Symbol
KLB
NCBI Official Synonym Symbols
BKL
NCBI Protein Information
beta-klotho
UniProt Protein Name
Beta-klotho
Protein Family
UniProt Gene Name
KLB
UniProt Synonym Gene Names
BKL; BetaKlotho
Uniprot Description
Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 4p14
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: 1-phosphatidylinositol-3-kinase activity; beta-glucosidase activity; fibroblast growth factor binding; fibroblast growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; Ras guanyl-nucleotide exchange factor activity; signal transducer activity
Biological Process: fibroblast growth factor receptor signaling pathway; MAPKKK cascade; phosphoinositide-mediated signaling; regulation of phosphoinositide 3-kinase cascade
Research Articles on KLB
Similar Products
Product Notes
The Human KLB klb (Catalog #AAA178734) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178734 ELISA Kit recognizes Human KLB. It is sometimes possible for the material contained within the vial of "Klotho beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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