Western Blot (WB)
(MBS635769 binds Recombinant Human FGF?21 in a functional ELISA. 50% of the optimal binding response for 200ng/well of recombinant human Klotho beta is observed at ~2.5-20ng/ml of recombinant human FGF-21.)
Klotho beta Active Protein | KLB active protein
Klotho beta, Recombinant, Human (Beta-klotho, BKL, BetaKlotho, Klotho beta-like Protein)
Gene Names
KLB; BKL
Purity
Highly Purified>95% by SDS-PAGE, reducing conditions and visualized with silver stain.
Synonyms
Klotho beta; Recombinant; Human (Beta-klotho; BKL; BetaKlotho; Klotho beta-like Protein); KLB active protein
Purity/Purification
Highly Purified
>95% by SDS-PAGE, reducing conditions and visualized with silver stain.
>95% by SDS-PAGE, reducing conditions and visualized with silver stain.
Form/Format
Supplied as a liquid in PBS, pH 6.8, 0.1mM EDTA, 50% glycerol.
Concentration
0.27mg/ml (varies by lot)
Application Notes
SDS-PAGE: 113-125kD, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Endotoxin Level
<1EU/ug (LAL)
N-terminal Sequence Analysis
Phe53
Specific Activity
When Recombinant human FGF-21 is immobilized at 1ug/ml (100ul/well) Recombinant human Klotho B His-tag binds with an ED50 of 2.5-20ng/ml
Preparation and Storage
Aliquot to avoid repeated freezing and thawing and store at -70 degree C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(MBS635769 binds Recombinant Human FGF?21 in a functional ELISA. 50% of the optimal binding response for 200ng/well of recombinant human Klotho beta is observed at ~2.5-20ng/ml of recombinant human FGF-21.)
Western Blot (WB)
(MBS635769 binds Recombinant Human FGF?21 in a functional ELISA. 50% of the optimal binding response for 200ng/well of recombinant human Klotho beta is observed at ~2.5-20ng/ml of recombinant human FGF-21.)
ELISA (EIA)
(Western Blot (reducing (R) conditions) analysis using MBS635769 (1ug/lane) and visualized by silver staining, showing a single band at 126kD.)
ELISA (EIA)
(Western Blot (reducing (R) conditions) analysis using MBS635769 (1ug/lane) and visualized by silver staining, showing a single band at 126kD.)
Related Product Information for KLB active protein
Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulindependent glucose uptake. The 1043aa type I transmembrane protein is composed of a 51aa signal sequence, a 943aa extracellular domain (ECD) containing two glycosidase-like regions, a 21aa transmembrane domain, and 28aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta-glycosidases, it was initially unclear whether they were functional enzymes. However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible. The extracellular domain shares 79%, 87%, 87% and 67% identity with mouse, equine, canine and rat Klotho beta, respectively. The low identity with rat reflects aa discordance within rodent ECD.
Recombinant protein corresponding to Phe53-Leu997 from human Klotho beta, with a C-terminal 10-His tag expressed in NSO cells. Swiss/UniProt Accession: Q86Z14.
Recombinant protein corresponding to Phe53-Leu997 from human Klotho beta, with a C-terminal 10-His tag expressed in NSO cells. Swiss/UniProt Accession: Q86Z14.
Product Categories/Family for KLB active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
110kD
NCBI Official Full Name
klotho beta
NCBI Official Synonym Full Names
klotho beta
NCBI Official Symbol
KLB
NCBI Official Synonym Symbols
BKL
NCBI Protein Information
beta-klotho; betaKlotho; klotho beta like; klotho beta-like protein
UniProt Protein Name
Beta-klotho
Protein Family
UniProt Gene Name
KLB
UniProt Synonym Gene Names
BKL; BetaKlotho
UniProt Entry Name
KLOTB_HUMAN
Uniprot Description
Klotho beta: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21. Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 4p14
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein binding; fibroblast growth factor binding; fibroblast growth factor receptor binding; hydrolase activity, hydrolyzing O-glycosyl compounds
Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of cell proliferation; carbohydrate metabolic process; insulin receptor signaling pathway; innate immune response
Research Articles on KLB
Similar Products
Product Notes
The KLB klb (Catalog #AAA635769) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. SDS-PAGE: 113-125kD, reducing conditions. Researchers should empirically determine the suitability of the KLB klb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Klotho beta, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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